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Scientifically Published Results on Longevity Studies Return to Homepage





The Longevity Lifespan Project involves taking the bulk of anti-aging studies in the medical and scientific arena and then where possible, locating foods and supplements that are high in these anti-aging minerals and vitamins.  In some cases compatible synergists are also included, so that you can take less. Note: Many anti-aging extracts work best when taken in small amounts over a long period of time. If too much is taken in a short period they have an opposite effect. In most cases causing cancer or other harm. In this case less is more.  In most cases,  you can eat foods high in these longevity substances and not suffer the same effects as foods provide a natural overdose protection. When eaten in moderation they can have better effects than some extracts.  The reserach is now starting to come together, showing that the proper foods are having the same effect as prolonging life as supplements, extracts and manufactured anti-aging drugs.

All Highlighted Text and text in italics is our researched foods and information that dovetail with the scientific research, so that a complete longevity diet can be discovered to extend the human lifespan.


All these papers have been summarized into a new book released November 2013.  The first 3 Chapters of the book are available for free on the book description page.



Herbal cocktail ka-mi-kae-kyuk-tang stimulates mouse
bone marrow stem cell






Yunnan Degao is a healthy food produced by Yunnan Delinhai Biological Sci-Tech Co.,Ltd.This product is prepared from eight precious ingredients of Chinese herbal medicines-Solomon's seal,fuling,Chinese wolfberry,Chinese yam,longan meat,walnut kernel,apricot kernel and chrysanthemum flower. For averaged maximum survivable time of adults,it was increased by 90.10%~124.22% in females and by 70.78%~114.14% in males.When adults were reared on complete Yunnan Degao diet,the averaged longevity of females were increased by 93.58%,and that of males by 84.5%,the survivable time of half amount of females was increased in average by 112.5%,and that of males by 108.7%.These results indicated that Yunnan Degao might have a powerful function in prolonging life of D.suzukii.
http://en.cnki.com.cn/Article_en/CJFDTOTAL-KCZS200704033.htm

Rats fed a diet exclusively of raw egg yolk grew well and were able to reproduce. Raw yolk was superior to cooked in promoting rat growth. The cooked whole egg supported much better rat growth than when fed raw. However, Adams (1964) reported that rats fed diets consisting of 100 percent cooked whole egg or cooked yolk had frequent diarrhea and food consumption was low with a resulting reduced growth rate and life span. When the yolk was supplemented with a salt mixture, weight gain, feed efficiency and longevity were greatly enhanced.< BR>http://ps.fass.org/content/45/4/788.short

Nutrients in Egg Yolk
http://nutritiondata.self.com/facts/dairy-and-egg-products/113/2

Vitamin A 3504IU 70%
Folate 355 mcg 89%
Vitamin B12 4.7 mcg 79%< BR>Pantothenic Acid 7.3 mg 73%
Choline 1658mg
Phosphorus 948 mg 95%
Selenium 136 mcg 194%


Vegetarians May Suffer Shorter Life Due to Carnitine, Carnosine, and Vitamin B12 Deficiencies
http://emediahealth.com/2011/04/20/vegetarians-may-suffer-shorter-life-due-to-carnitine-carnosine-and-vitamin-b12-deficiencies/

There is a readily available, eat-to-use source of vegan carnosine�just � teaspoon per day will give you 1000 mg. Vegans are low in B-12 and have almost no levels of Carnosine. That�s absolutely true. Meat-eaters are deficient, too. There is a minimum level of B-12 you need to survive and a maximum level that is optimal for long-
term health. At the minimum level, (180-200 nano-grams of B-12).
http://www.veganmainstream.com/2010/11/30/dr-gabriel-cousens-on-diet-deficiencies-for-vegans-and-meat-eaters/

Carnosine Synergists: Such substances include vitamin D 3, oleic and linoleic acids, blueberry extract, carnosine, fucoidan and blue green alge.
These combinations are already used to remove wrinkles:
http://www.nowfoods.com/Wrinkle-Rescue-Moisturizer-2oz.htm

Blueberry, green tea, vitamin D3, and carnosine, however, were found to increase bone marrow cell activity in a dose-dependent manner. For example, a combination of blueberry and vitamin D3 exhibited a 62% increase in proliferation of bone marrow cells.43 Blueberry and catechin (green tea extract) increased bone marrow cell proliferation by 70%. When carnosine and blueberry were combined, the growth promotion observed was 83% . . . an effect significantly greater than that of the expensive drug GM-CSF!43. A combination of blueberry, green tea, vitamin D3, and carnosine, however, increased these stem cells by an astounding 68%.

Stem Cell Therapy in a Pill?
http://www.lef.org/magazine/mag2006/dec2006_awsi_01.htm

The effect of carnosine on the life span and several brain biochemical characteristics in senescence-accelerated mice�prone 1 (SAMP1) was investigated. A 50% survival rate of animals treated with carnosine increased by 20% as compared to controls. Moreover, the number of animals that lived to an old age significantly increased.
http://online.liebertpub.com/doi/abs/10.1089/rej.1.1999.2.337

Oral supplementation with L-Carnosine resulted in demonstrable improvements in autistic behaviors as well as increases in language comprehension that reached statistical significance
https://www.1stvitality.co.uk/health/autism/

Carnosine may synergize with Alanine.

Supplementation with 1.8% carnosine and 1.2% beta-alanine resulted in markedly higher carnosine (up to +160%)
http://www.ncbi.nlm.nih.gov/pubmed/22895378

Foods highest in Alanine include:
Raw Seaweed, Egg White, Spirulina, Tuna and Pumpkin Seeds
http://nutritiondata.self.com/foods-000091000000000000000.html

For vegetarians who fastidiously adhere to a diet devoid of meat, their �Achilles� heel� may be lack of carnosine. This was confirmed in a paper published in October 2005 titled, �Glycation, ageing and carnosine: Are carnivorous diets beneficial?�41
http://www.lef.org/magazine/mag2006/jan2006_awsi_01.htm

We show here that feeding rapamycin to adult Drosophila produces the life span extension seen in some TOR mutants
http://www.ncbi.nlm.nih.gov/pubmed/20074526 

Rapamycin, also called sirolimus is produced by the soil bacterium Streptomyces hygroscopicus found on Easter Island. Sirolimus lowers the body's ability to fight an infection/disease (immunosuppression). This may increase your risk of developing an infection or certain types of cancer (e.g., skin cancer, lymphoma). To reduce the risk of these serious side effects, take this medication at the lowest effective dose.
This could prove that the immune system is adjusting to conditions.  Once these conditions have been adjusted to it can fight disease and bacteria more efficiently.
http://www.medicinenet.com/sirolimus_tablets-oral/article.htm

A plaque, written in Brazilian Portuguese, commemorating the discovery of sirolimus on Easter Island, near Rano Kau
A combination therapy of doxorubicin and sirolimus has been shown to drive AKT-positive lymphomas into remission in mice
Rapamycin was first shown to extend lifespan in eukaryotes in 2006.in a 2009 study, the lifespans of mice fed rapamycin were increased between 28 and 38% from the beginning of treatment, or 9 to 14% in total increased maximum lifespan. Of particular note, the treatment began in mice aged 20 months, the equivalent of 60 human years.
http://en.wikipedia.org/wiki/Sirolimus

 


Twelve Longevity Enhancement Methods Demonstrated in Mice
https://www.fightaging.org/archives/2009/08/a-list-of-interesting-longevity-enhancement-methods-in-mice.php

Nutrient 'Cocktail' Delays Aging and Extends Life Span
http://www.lef.org/magazine/mag2012/may2012_Nutrient-Cocktail-Delays-Aging-Extends-Life-Span_01.htm< /STRONG>

McMaster University makes cocktail to slow aging
 Using bagel bits soaked in the supplement to ensure consistent and
 accurate dosing, the formula maintained youthful levels of locomotor
 activity into old age whereas old mice that were not given the
 supplement showed a 50 per cent loss in daily movement.

Ingredients consists of items that were purchased in local stores
 selling vitamin and health supplements for people, including vitamins
 B1, C, D, E, acetylsalicylic acid, beta carotene, folic acid, garlic,
 ginger root, ginkgo biloba, ginseng, green tea extract, magnesium,
 melatonin, potassium, cod liver oil, and flax seed oil
http://www.longecity.org/forum/topic/38286-mcmaster-university-makes-cocktail-to-slow-aging/

A lack of Folic Acid, Niacin, VItamins B12, B6 and C during Late Winter and Early Spring may cause a shorter lifespan of those born in August and February
http://www.longevity-science.org/Season-of-Birth.pdf< /STRONG>

Effects of carnosine plus vitamin E and betaine treatments on
 oxidative stress in some tissues of aged rats.
 Carnosine (CAR) and betaine have antioxidant actions. The combination
 of CAR with vitamin E(CAR+E) increases its antioxidant efficiency
 (authors note: Synergy)
 These results suggest that CAR+E treatment seems to be useful to
 decrease oxidative stress in liver, heart and brain tissues, but
 betaine is only effective in liver tissue of aged rats.
 
http://www.ncbi.nlm.nih.gov/pubmed/23701646

Similarly,
 participants who consumed >360 mg/d of betaine had, on average, 10% lower
 concentrations of homocysteine (P < 0.01), 19% lower concentrations of
 C-reactive protein (P < 0.1), and 12% lower concentrations of tumor necrosis
 factor-alpha (P < 0.05) than did those who consumed <260 mg/d
 Participants with the largest increases in betaine
 concentrations showed a borderline significant (P = 0.07) higher memory
 performance compared to those without it.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=31794

Chronic treatment of (-)deprenyl prolongs the life span of male
http://www.ncbi.nlm.nih.gov/pubmed/8423709
 

Sexually low performing male rats die earlier than their high
 performing peers and (-)deprenyl treatment eliminates this
 difference
http://www.ncbi.nlm.nih.gov/pubmed/8152326

We found significant associations between month of birth and longevity: individuals born in September�November have higher likelihood of becoming centenarians compared to March-born individuals. Similar month-of-birth effects on longevity were found for centenarian spouses: individuals born in October-November were more likely to live to 100 compared to individuals born in April. The results of this study suggest that early life environmental conditions may have long-lasting effects on human aging and longevity.
http://longevity-science.org/pdf/Gavrilov-JAR-2011.pdf

Results showed that being overweight at a young age may significantly decrease chances of living to 100. Our results also supported previous findings that a farm upbringing improves survival to advanced ages. (Gavrilova and Gavrilov 2007; Preston, Hill, and Drevenstedt 1998).
http://longevity-science.org/pdf/Gavrilov-Biodemography-2012.pdf < /STRONG>

Experiments on laboratory mice found the offspring born to younger mothers live longer. These studies show better survival for people born in September through December compared to people born in the middle of the year. More specific explanation of the observed effects of early-life conditions on longevity can be provided by the inflammation hypothesis suggested by Finch and Crimmins (2004). According to this hypothesis, a strong acute-phase inflammatory response required for survival early in life initiates chronic inflammation, which promotes chronic diseases of aging. The results obtained in our study suggest that optimizing the process of early-development can potentially result in avoiding many diseases in later life and significantly extending healthy life span.
http://longevity-science.blogspot.com/2011/02/international-conference-living-to-100.html < /STRONG>

In order to explain the secular decline in mortality due to cardiovascular illnesses, Finch and Crimmins (2004) propose that exposure to infectious diseases early in life increases the risk of chronic inflammation through life course. This paper seeks evidence for this hypothesis with data from a Costa Rican aging study, called CRELES.
http://ccp.ucr.ac.cr/creles/pdf/brenespaa07.pdf < /STRONG>

It was found that the ��stout�� body build (being in the heaviest 15% of control population) was negatively associated with longevity and persons with slender and medium body build had higher chances of survival to 100 (OR�2.63, p�0.025). Both farming occupation and having large number of children (4�) at age 30 significantly increased the chances of exceptional longevity (OR�2.20, p�0.016 and OR�2.71, p�0.051, respectively).
http://longevity-science.org/Centenarians-RR-2010.pdf< /STRONG>

Another interesting result of our study is a positive and significant effect of farming on survival to age 100. This result is consistent with our previous findings suggesting that children < /FONT>raised on farms (boys in particular) had higher chances to become centenarians (Gavrilova and Gavrilov, 2007).
http://paa2009.princeton.edu/papers/91719 < /STRONG>

Melatonin Extends Lifespan
http://www.lef.org/magazine/mag95/95dec2d.htm
Foods highest in Melatonin
Tart (sour) cherry juice concentrate 17,535
Cherry Juice helps you sleep better

Latest studies focus on carnosine & longevity
http://www.examiner.com/article/latest-studies-focus-on-carnosine-longevity  

Remarkable gerontoprotective effect of LPBNAH are demonstrated after chronic administration �with extension of mean and maximal lifespan by more than 250%, indicating that this amphiphilic antioxidant agent is far superior to all other previously tested antioxidant compounds and at least one order of magnitude more potent than any previously documented anti-ageing strategies
http://www.markus-maute.de/aginglinks2.html
http://www.ncbi.nlm.nih.gov/pubmed/12044951?dopt=Abstract< /STRONG>

The reason why membrane fatty acid composition is correlated with MLSP is because fatty acids differ greatly is their susceptibility to lipid peroxidation. Only polyunsaturated fatty acids undergo peroxidation (saturates and monounsaturates are peroxidation-resistant), and the more polyunsaturated the fatty acid, the greater its peroxidation susceptibility. membrane fatty acid composition to maximum lifespan
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082016/ < /STRONG>

Much evidence has indicated that beta-carotene and retinol may be important natural anticarcinogens. Accordingly, they also may be important antiaging agents. This possibility has been tested by determining if a positive correlation exists between the concentration of carotenoids and retinol in serum and brain tissue with the maximal life-span potential of mammalian species. The results show a significant positive correlation for the carotenoids but not for retinol. These results suggest that the carotenoids may be biologically active as protective agents against cancer and as longevity determinants. Retinol appears to be less important in these functions.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC392201/< /STRONG>

Remarkably, centenarians were characterized as having the highest levels of vitamins A and E, whereas the activities of both plasma and RBC SOD, which increase with age, decreased in centenarians. From these results, it is evident that healthy centenarians show a particular profile in which high levels of vitamin A and vitamin E seem to be important in guaranteeing their extreme longevity.
http://www.ncbi.nlm.nih.gov/pubmed?cmd=Retrieve&list_uids=10889454&dopt=AbstractPlus

Foods Highest in Vitamin A
Broccoli Leaves, Carrots, Mustard Spinach, Lettuce, Kale
http://nutritiondata.self.com/foods-000098000000000000000-1.html

 

If treated with the catalytic antioxidant MnTBAP, the life span of the mice can be extended, and the liver and heart pathology can be prevented. Catalytic antioxidants mimic the activities of superoxide dismutase or catalase or both, detoxifying superoxide and hydrogen peroxide, and in some cases, peroxynitrite and other toxic species as well. Several compounds have demonstrated efficacy in in vitro and in animal models of neurodegeneration, leading to optimism that catalytic antioxidants may prove to be useful therapies in human disease.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2933572/< /STRONG>

Substances That Interfere With High Performance Catalysts. Many substances affect both the absorption and loss of high performance catalysts. For example, the amount absorbed from foods can be influenced by dietary constituents (such as fiber), other factors (such as medications), the body�s need for the nutrient, the chemical form of the nutrient, and the integrity of the digestive tract. �ANTI-catalysts� that can interfere with how well the body uses nutrients are shown below.< /STRONG>http://www.usuhs.mil/mem/wng/WNGChapter4.pdf< /STRONG>

The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide
http://www.nutritionj.com/content/9/1/3< /STRONG>

PBN extends the life-span of SAM-P8 (senescence accelerated mouse) mice approximately 33% when administered via intraperitoneal (IP) injections starting at the age of 3 months
http://www.uwaging.org/genesdb/gene.php?id=259< /STRONG>

We administered a small-molecule synthetic enzyme superoxide dismutase (SOD) mimetic to wild-type (i.e. non-transgenic, non-senescence accelerated) mice starting at middle age. Chronic treatment not only reduced age-associated oxidative stress and mitochondrial radical production, but significantly extended lifespan
http://www.ncbi.nlm.nih.gov/pubmed/17079053?dopt=AbstractPlus < /STRONG>

Blueberry polyphenols increase lifespan
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1413581/?tool=pubmed< /STRONG>

Sirtuin activating compounds (STACs) can promote the survival of human cells and extend the replicative lifespan of yeast. Here we show that resveratrol and other STACs activate sirtuins from Caenorhabditis elegans and Drosophila melanogaster, and extend the lifespan of these animals without reducing fecundity. Lifespan extension is dependent on functional Sir2, and is not observed when nutrients are restricted. Together these data indicate that STACs slow metazoan ageing by mechanisms that may be related to caloric restriction.
http://www.ncbi.nlm.nih.gov/pubmed/15254550< /STRONG>

The chronic treatment of female C3H/Sn mice with phenformin prolonged the mean life span by 21.1% (P < 0.05), the mean life span of the last 10% survivors by 28.4% and the maximum life span by 5.5 months (by 26%) in comparison with the control.
http://www.ncbi.nlm.nih.gov/pubmed/14618027< /STRONG>

We identified 3 pharmacologic compounds, ethosuximide, trimethadione, and 3,3-diethyl-2-pyrrolidinone, that extend lifespan and delay age-related degenerative changes in the nematode worm Caenorhabditis elegans. All 3 compounds are anticonvulsants that modulate neural activity in vertebrates, and ethosuximide and trimethadione are used to treat absence seizures in humans. We discuss existing evidence that these drugs might also delay vertebrate aging and suggest experiments that could test this hypothesis.
http://www.ncbi.nlm.nih.gov/pubmed/16606760< /STRONG>

Resveratrol was added to the food starting in early adulthood and caused a dose-dependent increase of median and maximum lifespan.
http://www.ncbi.nlm.nih.gov/pubmed/16461283

Blueberries, Grapes and Cacao Chocolate are high in resveratrol
http://www.livescience.com/39125-foods-good-sources-resveratrol.html

These lower levels of oxidized guanines are associated with increased longevity and hMTH1-Tg animals live significantly longer than their wild-type littermates

http://www.ncbi.nlm.nih.gov/pubmed/23648059

Results revealed that blueberry extracts at 5mg/ml in diet could significantly extend the mean lifespan of fruit flies by 10%, accompanied by up-regulating gene expression of superoxide dismutase (SOD), catalase (CAT) and Rpn11 and down-regulating Methuselah (MTH) gene
http://www.ncbi.nlm.nih.gov/pubmed/22197903

Mesenchymal stromal cells prolong the lifespan in a rat model of amyotrophic lateral sclerosis
http://www.ncbi.nlm.nih.gov/pubmed/21736505

We found that supplementing starved larvae with ethanol markedly extends their lifespan within this L1 diapause.
http://www.ncbi.nlm.nih.gov/pubmed/22279556

Ethanol Content in Foods:
http://jat.oxfordjournals.org/content/22/3/181.full.pdf

These results indicated that dietary RJ increased the average life span of C3H/HeJ mice, possibly through the mechanism of reduced oxidative damage
http://www.ncbi.nlm.nih.gov/pubmed/12954483

We demonstrate that the lifelong consumption of a diet rich in omega-3 fatty acids prolongs murine reproductive function into advanced maternal age, while a diet rich in omega-6 fatty acids is associated with very poor reproductive success at advanced maternal age. Thus, omega-3 fatty acids may provide an effective and practical avenue for delaying ovarian aging and improving oocyte quality at advanced maternal age
http://www.ncbi.nlm.nih.gov/pubmed/22978268

Foods highest in Omega 3 Fatty Acids
Flaxseed Oil and Seeds, Chia Seeds
http://nutritiondata.self.com/foods-000140000000000000000.html

Here, we show that CoQ(10) and Vitamin E extended the life span of wild-type Caenorhabditis elegans.
http://www.ncbi.nlm.nih.gov/pubmed/14706236

Supplementing C. elegans culture media with these ω-6 PUFAs increases their resistance to starvation and extends their life span in conditions of food abundance
http://www.ncbi.nlm.nih.gov/pubmed/23392608

Fats and oils, meat and poultry, cereal-based products and cereals, vegetables, and nuts and seeds were important sources of n-6 PUFA.
�PUFA Oils in Processed Foods
 Corn oil: 54.5% Omega-6 PUFA
Sunflower oil: 68%
�Vegetable oil (Look at the ingredients, there�s only one: soybean oil.): 51.4%
�Cottonseed oil: 52.4%
 �Grapeseed oil: 70.6% Omega-6 PUFA (can I get a �holy-freaking-CRAP!?�)
�Walnut oil: 53.9%
 
http://butterbeliever.com/what-is-pufa/

The life span of Drosophila melanogaster (Oregon R) males was found to be proportional to the logarithm of the iron content of the diet. Life span was also shown to be proportional to the rate of iron accumulation for Drosophila, mice and man. The total body iron content was found to correlate with the total calcium content of adult Drosophila. Iron content during the developmental stages, however, remained relatively constant and did not change with changes in the calcium concentrations. Dietary tea (Camellia sinensis) extracts were found to inhibit the ageing-related accumulation of iron and to prolong the life span of Drosophila by as much as 21.4%. It is concluded that iron accumulation is a significant factor contributing to senescence.
http://www.ncbi.nlm.nih.gov/pubmed/8326745

Foods that remove excess iron;
green tea, IP6 rice bran extract, Cell Forte or Jarrow brand of IP6,
http://www.healthyprotocols.net/_membersfiles/WEB%20Files/Working%20WEB%20Files/3_iron_1.htm

Apple polyphenols extend the mean lifespan of Drosophila melanogaster. Results showed the mean lifespan was significantly extended by 10% in fruit flies fed the AP diet.
http://www.ncbi.nlm.nih.gov/pubmed/21319854

Vitamins May Shorten Not Prolong Lifespan: Danish study in JAMA
http://forums.randi.org/archive/index.php/t-75950.html

The following supplements have been shown to extend lifespan or reduce age-related damage in senescence accelerated mice:Abalone Mushroom, Acetyl-L-Carnitine, Aged Garlic, American Ginseng, Asiaticoside, Beta-Cryptoxanthin, Chinese Mahogany, Coral Calcium, Curcumin, DHEA, Forsythiaside, Ginsenoside, Green Tea Catechins, Melatonin, Mulberry, Nobiletin, Olive Oil, Onion, Panax ginseng, Persimmon, Pu-erh Tea, Pterostilbene, Purple Sweet Potato, Pycnogenol, Red Bell Pepper, Resveratrol, Rose Flower, Spirulina.
http://www.lef.org

Curcumin found in turmeric and mustard oxidizes to vanillin. Curcumin synergizes with Curcuma longa and cysplatin

Aged garlic extract prolongs longevity and improves spatial memory deficit in senescence-accelerated mouse
http://www.ncbi.nlm.nih.gov/pubmed/8850329

How to Make Aged Garlic
http://www.ehow.com/how_5671985_make-aged-garlic.html

Extending life span by increasing oxidative stress
http://www.ncbi.nlm.nih.gov/pubmed/21619928

We found a very significant increase in average longevity (17%) of the cyclists when compared with the general population. The age at which 50% of the general population died was 73.5 vs. 81.5 years in Tour de France participants. Our major finding is that repeated very intense exercise prolongs life span in well trained practitioners. Our findings underpin the importance of exercising without the fear that becoming exhausted might be bad for one's health.
http://www.ncbi.nlm.nih.gov/pubmed/21618162

This research found that older individuals with more positive self-perceptions of aging, measured up to 23 years earlier, lived 7.5 years longer than those with less positive self-perceptions of aging
http://www.ncbi.nlm.nih.gov/pubmed/12150226

As the quartile ranking of positive emotion in early life increased, there was a stepwise decrease in risk of mortality resulting in a 2.5-fold difference between the lowest and highest quartiles. Positive emotional content in early-life autobiographies was strongly associated with longevity 6 decades later.
http://www.ncbi.nlm.nih.gov/pubmed/11374751

Overexpression of sirtuins (NAD+-dependent protein deacetylases) has been reported to increase lifespan in budding yeast (Saccharomyces cerevisiae), Caenorhabditis elegans and Drosophila melanogaster1
http://www.nature.com/nature/journal/v477/n7365/abs/nature10296.html

< P>Frthermore, EGb 761 increased the wild type's resistance to acute oxidative and thermal stress by 33% and 25%, respectively. Treatment of the prematurely aging mutant worms mev-1 with EGb 761 increased their resistance to acute oxidative and thermal stress by 33% and 11%, respectively. It appears that oxidative stress, a major determinant of life span, as well as other types of stress, can be successfully counteracted by the Ginlkgo biloba extract EGb 761.
http://www.ncbi.nlm.nih.gov/pubmed/12396085

For example, we used zebrafish genes to alter mitochondrial function and innate immunity in ways not normally available to C. elegans and extended worm lifespan by ~40%. Next, we used a modular approach to extend lifespan by 130% by combining up to four components in the same strain. These results provide a platform to build worms having progressively longer lifespans. This project is conceptually similar to using engineering to increase the useful lifespan of a primitive machine (1931 Model T) using both parts from the model T as well as parts from a more advanced machine (2012 Toyota Corolla). Our results open the door to use engineering to go beyond the constraints of the C. elegans genome to extend its lifespan by adding non-native components.
http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002780

< P>Bacterial nitric oxide extends the lifespan of C. elegans
http://www.ncbi.nlm.nih.gov/pubmed/23415229< /STRONG>

Instead, Dr. Nudler and his team report in the February 14th online issue of Cell that the worm can "hijack" the compound from the soil-dwelling Bacillus subtilis bacterium that is not only a favored food but also a common colonist within its gut. This resourcefulness, Dr. Nudler says, partially explains why worms fed B. subtilis live roughly 50 percent longer
http://www.sciencedaily.com/releases/2013/02/130214132623.htm

The documented consumption of B. subtilis in the Japanese fermented soy bean, natto. Natto, which is commonly consumed in Japan, contains as many as 108 viable B. subtilis per gram
http://en.wikipedia.org/wiki/Bacillus_subtilis

Natto just happens to be one of the foods highest in Vitamin K.
We propose that the viable probiotic characteristics of Bacillus subtilis natto benefit calf immune function.

Effects of Bacillus subtilis natto on performance and immune function of preweaning calves
http://www.ncbi.nlm.nih.gov/pubmed/21094758

Effect of the tocotrienol-rich fraction on the lifespan and oxidative biomarkers in Caenorhabditis elegans under oxidative stress. The tocotrienol-rich fraction restored the lifespan of oxidative stress-induced C. elegans and reduced the accumulation of lipofuscin
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654308/

Low-dose lithium uptake promotes longevity in humans and metazoans
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151375/< /STRONG>

Foods or food sources rich in lithium include all kinds of dairy products, sugarcane, seaweed, potatoes, lemons and eggs. Natural mineral water is also said to have a good source of lithium
http://www.diethealthclub.com/blog/diet-profile/foods-rich-in-lithium-and-lithium-supplements.html

Lithium-Rich Foods
Milk                                7533
Eggs                               7373
Pork                               3844
Tomatoes                       6707
Mushrooms                    5788
Cucumbers                     5017
Black Tea                        3737
Paprika                           2316
Instant Soups                 2513
Rice                                1260
http://collectivewizdom.com/Lithium-LithiumRichFoods.html

A regular uptake of the trace element lithium can considerably promote longevity. This is the result of a new study by scientists of Friedrich Schiller University Jena
http://www.sciencedaily.com/releases/2011/02/110218111709.htm< /STRONG>

The 30 Nutrients Used in This Health and Longevity Study
 Vitamin B1, Vitamin B3 (niacin), Vitamin B6, Vitamin B12, Folate , Vitamin C, Vitamin D, Acetyl-L-carnitine, Alpha-lipoic acid, Acetylsalicylic acid (aspirin), Beta-carotene, Bioflavonoids, Chromium picolinate, Garlic, Ginger root extract, Ginkgo biloba, Ginseng, Green tea extract, L-Glutathione,  Magnesium, Manganese, Melatonin, N-Acetyl cysteine, Potassium, Rutin, Selenium, Vitamin E, Cod liver oil (omega-3), Coenzyme Q10, Flax seed oil

Source:
http://www.lef.org/magazine/mag2012/may2012_Nutrient-Cocktail-Delays-Aging-Extends-Life-Span_01.htm

< P>In contrast, α-lipoic acid, but not EGCG, extended mean and maximal life span in C. elegans
http://www.sciencedirect.com/science/article/pii/S0091305706003522

Sources of Alpha-Lipoic Acid

�Spinach
�Kale (Kale is also superhigh in Vitamin K)
�Collards
�Broccoli
http://brotherecho.com/2012/07/03/alpha-lipoic-acid/

 

< P>Abstract Vitamin E extends the lifespan of many animals, including the nematode
Caenorhabditis elegans. Our results confirm previous studies that 200 μg/ml vitamin E
significantly prolonged C. elegans survival
http://www.sciencedirect.com/science/article/pii/004763748890098X

Foods highest in Vitamin E:
Quaker Oats,  Wheat Germ, Spinach, Chicory, Swiss Chard, Chrysanthemum,
http://nutritiondata.self.com/foods-000103000000000000000.html

< P>Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Note: Green Tea should be used in moderation and not excesively for best results.
http://www.sciencedirect.com/science/article/pii/S089158490800662X

< P>Quercetin mediated lifespan extension in Caenorhabditis elegans is modulated by age-1, daf-2, sek-1 and unc-43
http://link.springer.com/article/10.1007/s10522-008-9199-6

This further substantiates the epidemiological data suggesting that those who preferentially consume high amounts of quercetin-containing foods have a reduced risk of thrombosis and potential CVD risk.
http://www.ncbi.nlm.nih.gov/pubmed/16925853
 
In red onions, higher concentrations of quercetin occur in the outermost rings and in the part closest to the root
http://en.wikipedia.org/wiki/Quercetin

The polyphenol quercetin has recently been found to extend lifespan and increase stress resistance in the nematode Caenorhabditis elegans. We show that quercetin exposure leads to increased mean lifespans up to 15%. Furthermore, quercetin-treated daf-16(mgDf50) worms show an enhanced resistance to thermal and oxidative stress. Like green tea, quercetin should also be used in moderation.
http://www.sciencedirect.com/science/article/pii/S0047637408001607

Foods containing quercetin
Quercetin (mg/100g of edible portion)
capers 180[6]
lovage 170[7]
dock like sorrel 86[8]
dill 55[9] (48-110)[7]
onion, red 32[10]
In red onions, higher concentrations of quercetin occur in the outermost rings
http://en.wikipedia.org/wiki/Quercetin

In Greek popular medicine, a herbal tea made of caper root and young shoots is considered beneficial against rheumatism. Dioscoride (MM 2.204t) also provides instructions on the use of sprouts, roots, leaves and seeds in the treatment of strangury and inflammation.
http://en.wikipedia.org/wiki/Caper

< P>It is proposed that catechin modulates an energy-intensive stress response and repair system that results in reduced body length and an enhanced lifespan
http://www.sciencedirect.com/science/article/pii/S0047637409000724

< P>These results suggest that quercetin may act as an antioxidant as well as a modulator of cellular signalling processes to exert its protective properties.
http://www.sciencedirect.com/science/article/pii/S0300483X0700100X

< P>By testing drugs that are Food and Drug Administration approved for human use, we discovered that the mood stabilizer and anticonvulsant valproic acid (VA) extended C. elegans lifespan. VA also delayed age-related declines of body movement, indicating that VA delays aging. Valproic acid is a small carboxylic acid that is the most frequently prescribed anticonvulsant drug in humans. A structure�activity analysis demonstrated that the related compound valpromide also extends lifespan.< BR>http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2008.00375.x/full

< P>This study shows that exposure to low concentrations of the polyphenol tannic acid
(TA) induces potent life-prolonging properties in Caenorhabditis elegans.
http://www.ncbi.nlm.nih.gov/pubmed/20413530

Foods containing phenolics/tannins

Guarana 85,000-120,000 ppm
Black walnut 147,000 ppm
English walnut 122,300 ppm
Acorns contain such high concentrations of tannins that they must be processed before they can be consumed safely.
http://www.widomaker.com/~jnavia/tannins/tannlist.htm

When soaking walnuts, the tannins are rinsed away.
http://vegetarian.about.com/od/beverage1/a/soaking-nuts.htm

The extension of lifespan appeared to occur through a mechanism related to dietary restriction (DR), the reduction of available nutrients without causing malnutrition, an intervention that extends lifespan in diverse organisms from yeast to mammals
ht
tp://www.sciencedirect.com/science/article/pii/S0047637407001212

< P>Here, we report that reserpine largely known as an antipsychotic�antihypertensive drug, can extend C. elegans lifespan.
http://link.springer.com/article/10.1007/s10522-008-9139-5#page-1

< P>We examined the effects of curcumin on the lifespan and aging in Caenorhabditis elegans, and found that it responded to curcumin with an increased lifespan and reduced intracellular reactive oxygen species and lipofuscin during aging. Note: It is key to use organic curcumin, which gets best results.
http://www.sciencedirect.com/science/article/pii/S0047637411001187

< P>Abstract Extracts of plant adaptogens such as Eleutherococcus senticosus (or Acanthopanax
senti- cosus) and Rhodiola rosea can increase stress resistance in several model systems. We now show that both extracts also increase the mean lifespan of the nematode C. elegans.
http://link.springer.com/article/10.1007/s10522-008-9151-9#page-1

< P>In contrast, α-lipoic acid, but not EGCG, extended mean and maximal life span in C. elegans
http://www.sciencedirect.com/science/article/pii/S0091305706003522

< P>Abstract Vitamin E extends the lifespan of many animals, including the nematode
Caenorhabditis elegans. Our results confirm previous studies that 200 μg/ml vitamin E
significantly prolonged C. elegans survival
http://www.sciencedirect.com/science/article/pii/004763748890098X

< P>Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Note: Green Tea should be used in moderation and not excesively for best results.
http://www.sciencedirect.com/science/article/pii/S089158490800662X

< P>Quercetin mediated lifespan extension in Caenorhabditis elegans is modulated by age-1, daf-2, sek-1 and unc-43
http://link.springer.com/article/10.1007/s10522-008-9199-6

The polyphenol quercetin has recently been found to extend lifespan and increase stress resistance in the nematode Caenorhabditis elegans. We show that quercetin exposure leads to increased mean lifespans up to 15%. Furthermore, quercetin-treated daf-16(mgDf50) worms show an enhanced resistance to thermal and oxidative stress. Like green tea, quercetin should also be used in moderation.
http://www.sciencedirect.com/science/article/pii/S0047637408001607

< P>It is proposed that catechin modulates an energy-intensive stress response and repair system that results in reduced body length and an enhanced lifespan
http://www.sciencedirect.com/science/article/pii/S0047637409000724

< P>These results suggest that quercetin may act as an antioxidant as well as a modulator of cellular signalling processes to exert its protective properties.
http://www.sciencedirect.com/science/article/pii/S0300483X0700100X

< P>By testing drugs that are Food and Drug Administration approved for human use, we discovered that the mood stabilizer and anticonvulsant valproic acid (VA) extended C. elegans lifespan. VA also delayed age-related declines of body movement, indicating that VA delays aging. Valproic acid is a small carboxylic acid that is the most frequently prescribed anticonvulsant drug in humans. A structure�activity analysis demonstrated that the related compound valpromide also extends lifespan.< BR>http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2008.00375.x/full

< P>Pharmacogenetic analysis of lithium-induced delayed aging in Caenorhabditis elegans
Abstract Quercetin, Caffeic- and Rosmarinic acid exposure extend lifespan in Caenorhabditis
elegans.< BR>
http://link.springer.com/article/10.1007/s10522-011-9334-7

http://scholar.google.com/scholar?start=30&q=related:zuSEnr4CW32uBM:scholar.google.com/&hl=en&as_sdt=0,5

< P>Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans
http://www.sciencedirect.com/science/article/pii/S0047637412001303

Tyrosol, a main phenol present in extra virgin olive oil, increases lifespan and stress resistance in Caenorhabditis elegans
http://www.sciencedirect.com/science/article/pii/S0047637412001303

Tyrosol is found in Sherry, Extra Virgin Olive Oil, Vinegar and Black Raw Olives
http://www.phenol-explorer.eu/contents/polyphenol/673

Synergism between tyrosol and gallic acid isolated from Olive Mill Wastewater showed antimicrobial activity, at 100 �g/mL, against Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli,
http://www.scielo.br/scielo.php?pid=S0102-695X2013000200026&script=sci_arttext

Foods high in gallic acid:

Apple Cider Vinegar - some of the most potent beauty remedies contain a mixture of oilve oil and apple cider vinegar.
Rooibos Red Tea and/or Extracts
Pomegranate, pure juice

 

< P>The same heat or oxygen pretreatment regimens that induced subsequent stress resistance also increased life expectancy and maximum life span of populations undergoing normal aging
http://www.ncbi.nlm.nih.gov/pubmed/11867647

Protocatechuic acid extends lifespan and increases stress resistance in Caenorhabditis elegans
http://link.springer.com/article/10.1007/s12272-013-0183-6#page-1

A�a� oil, obtained from the fruit of the a�a� palm (Euterpe oleracea), is rich in protocatechuic acid (630 � 36 mg/kg).,[10] Protocatechuic acid also exists in the skins of some strains of onion as an antifungal mechanism, increasing endogenous resistance against smudge fungus. It is also found in Allium cepa (17,540 ppm).PCA occurs in roselle (Hibiscus sabdariffa), which is used worldwide as a food and beverage. Protocatechuic acid is also found in mushrooms such as Agaricus bisporus[12] or Phellinus linteus.
http://en.wikipedia.org/wiki/Protocatechuic_acid

< P>Sirtuin activators mimic caloric restriction and delay ageing in metazoans
http://www.nature.com/nature/journal/v430/n7000/abs/nature02789.html

There have been studies assessing the ability of different nutritive supplements to induce Sirt1 activity. Sirt1 mRNA levels were shown to increase in adipose tissue with a sixteen-week treatment of a combination of ephedrine, caffeine and the anti-diabetic drug Pioglitazone in nondiabetic human subjects (Bogacka, et al. 2007).When autoimmune prone (NZBxNZW)F1 mice were fed either a 5% corn oil or 5% fish oil (FO) AL diet, a significantly increased lifespan was noted in the FO-fed mice.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2727669/

< P>Exposure to the assayed flavonols also increased significantly the resistance against thermal and juglone-induced oxidative stress, although differences were found depending on the stage of development of the worm. Thus, quercetin offered greater protection when thermal stress was applied in the 1st day of adulthood, whereas tamarixetin was more efficient in worms submitted to stress in the 6th day of adulthood. Similarly, significantly greater protection was provided by quercetin than by its methylated derivatives at the 1st day of adulthood, whilst quercetin and isorhamnetin were equally efficient when the oxidative stress was induced in the 6th of day of adulthood.< BR>http://pubs.rsc.org/en/content/articlelanding/2011/fo/c1fo10049a/unauth#!divAbstract

< P>We demonstrate here that SQDB significantly extend life span in a C. elegans model of aging. Among all individual herbs tested, two herbs Cinnamomum cassia bark (Chinese pharmaceutical name: Cinnamomi Cortex, CIN) and Panax ginseng root (Chinese pharmaceutical name: Ginseng Radix, GS) significantly extended life span in C. elegans. .In this study, two commonly used traditional herbal formulas, Shi Quan Da Bu Tang (SQDB) and Huo Luo Xiao Ling Dan (HLXL) were evaluated using a survival assay and oxidative stress biomarkers in a well-established C. elegans model of aging
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0009339

Substances in this Formula (12 Total) for Shi Quan Da Bu Tang:
Ren Shen (Ginseng Root) 人参 6-9 grams

Bai Zhu (White Atractylodes Rhizome) 白术
9-12 grams

Fu Ling (Sclerotium of Tuckahoe, China Root, Poria, Hoelen) 茯苓
12-15 grams
Zhi Gan Cao (Honey Fried Licorice Root) 炙甘草
3-6 grams

Shu Di Huang (Chinese Foxglove Root, Steamed with Water or Alcohol, Rehmannia) 熟地黄
15-18 grams

Bai Shao (White Peony Root) 白芍
12-15 grams

Dang Gui (Chinese Angelica Root) 当归
12-15 grams

Chuan Xiong (Szechuan Lovage Root) 川芎
6-9 grams

Rou Gui (Inner Bark of Saigon Cinnamon) 肉桂
6-9 grams

Huang Qi (Astragalus) 黄芪
15-18 grams

Da Zao (Date, Jujube) 大枣
2 pieces
http://www.sacredlotus.com/formulas/get.cfm/chinese_formula/shi_quan_da_bu_tang


Substances in this Huo Luo Xiao Ling Dan Formula


Dang Gui (Chinese Angelica Root) 当归
15 grams

Dan Shen (Salvia Root) 丹参
15 grams

Ru Xiang (Frankincense Resin) 乳香
15 grams

Mo Yao (Myrrh) 没药
15 grams

 

< P>Blueberry extract prolongs lifespan of Drosophila melanogaster
http://www.sciencedirect.com/science/article/pii/S053155651100341X

< P>Dissolved humic substances facilitate fish life in extreme aquatic environments and have the potential to extend the lifespan of Caenorhabditis elegans
http://www.researchgate.net/profile/Thomas_Meinelt/citations/

The correlation between longevity and stress resistance observed in long-lived
mutant animals suggests that the ability to sense and respond to environmental challenges
could be important for the regulation of life span
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC329286/

Bushenkangshuai Tang (补肾抗衰方, BT) is a traditional Chinese medicine widely used for clinically treating premature ovarian failure. In the present study, BT administration at high concentrations significantly increased lifespan
http://link.springer.com/article/10.1007/s11684-009-0002-0#

Bushenkangshuai Tang formula:
Semen Cuscutae (Tusizi) 15 g,
Rhizoma Polygonati Sibirici (Huangjing) 15 g,
prepared Radix Rehmanniae (Shudihuang) 15 g,
Herba Cynomorii Songarici (Roucongrong) 10 g,
 Radix Morindae Officinalis (Bajitian) 10 g,
Radix Angelicae Sinensis (Danggui) 10 g,
Rhizoma Chuanxiong (Chuanxiong) 6 g,
Fluoritum (Zishiying) 15 g,
and Fructus Schisandrae Chinensis (Wuweizi) 6 g,
http://link.springer.com/article/10.1007/s11684-009-0002-0#

Curcumin Extends Life Span, Improves Health Span, and Modulates the Expression of Age-Associated Aging Genes in Drosophila melanogaster
http://online.liebertpub.com/doi/abs/10.1089/rej.2010.1031

Trehalose extends longevity in the nematode Caenorhabditis elegans. Trehalose is a disaccharide of glucose found in diverse organisms and is suggested to act as a stress protectant against heat, cold, desiccation, anoxia, and oxidation. Here, we demonstrate that treatment of Caenorhabditis elegans with trehalose starting from the young-adult stage extended the mean life span by over 30% without any side effects. Surprisingly, trehalose treatment starting even from the old-adult stage shortly thereafter retarded the age-associated decline in survivorship and extended the remaining life span by 60%.
http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2010.00582.x/full

Trehalose is available as an extract on amazon.com and is high in the foods honey, wine and mushrooms. It is especially high in foods that have been fermented using brewers yeast.

< P>Here, we used the Mexican fruit fly to screen 14 compounds and botanicals for their prolongevity effects and found an oregano and cranberry mixture (OC) improved survival
The greatest increase in longevity was observed in females fed the SY24:1 diet with 2% OC< BR>
http://www.ncbi.nlm.nih.gov/pubmed/19906819

Cranberry and oregano make seafood safe to eat
He found that using a 50:50 mixture of cranberry and oregano to coat cod fillets and shrimps infected with V. parahaemolyticus was far more effective at killing the bugs than either of the compounds on its own. The mixture became even more effective when lactic acid was added to it
http://www.newscientist.com/article/mg18825205.000-cranberry-and-oregano-make-seafood-safe-to-eat.html

Life span extension of Caenorhabditis elegans by novel pyridoperimidine derivative.
http://www.ncbi.nlm.nih.gov/pubmed/22297744

Here, we report that celecoxib, a nonsteroidal anti-inflammatory drug widely used to treat pain and inflammation, extends Caenorhabditis elegans lifespan and delays the age-associated physiological changes, such as motor activity decline. Celecoxib also delays the progression of age-related proteotoxicity as well as tumor growth in C. elegans
http://www.ncbi.nlm.nih.gov/pubmed/21348927

Nectarine promotes longevity in Drosophila melanogaster
http://www.sciencedirect.com/science/article/pii/S0891584911001687

< P>Under what nutritional conditions does resveratrol affect lifespan and reproduction?
Only under one diet combination, resveratrol appears to increase mean lifespan< BR>
http://www.sciencedirect.com/science/article/pii/S0531556509000369

< P>A�ai palm fruit ( Euterpe oleracea Mart.) pulp improves survival of flies on a high fat diet
http://www.sciencedirect.com/science/article/pii/S0531556510000422

< P>Supplementation of the diet with cranberry extract (CBE) increased the lifespan in C. elegans in a concentration-dependent manner.
http://link.springer.com/article/10.1007/s11357-012-9459-x#

The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster
Resveratrol at 400 μM extended lifespan of females fed a high-fat diet.
http://link.springer.com/article/10.1007/s11357-011-9332-3#page-2

< P>Aloe vera or resveratrol supplementation in larval diet delays adult aging in the fruit fly, Drosophila melanogaster
http://www.ncbi.nlm.nih.gov/pubmed/21719611

Dietary activators of Sirt1
http://www.ncbi.nlm.nih.gov/pubmed/19010386

Vitamin E may affect the life expectancy of men, depending on dietary vitamin C intake and smoking
http://www.ncbi.nlm.nih.gov/pubmed/21242192

A botanical containing freeze dried a�ai pulp promotes healthy aging and reduces oxidative damage in sod1 knockdown flies
http://link.springer.com/article/10.1007/s11357-012-9437-3#

Nectarines, resveratrol and Aloe vera extract supplementation of diet extended adult longevity in Drosophila melanogaster.  Vitamin E may extend the life expectancy of men with 2 years, if they have a dietary vitamin C intake above the median . dietary supplementation, for 15 weeks, with 5% and 20% (w/w) of nutritional doses of vitamins C and E, zinc, selenium, and beta-carotenes restored redox homeostasis and improved leukocyte functions,. Nitrone-based free radical trapping agents. α-phenil-tert-butyl nitrone (PBN) and other nitrone-based free radical trapping agents prolong both the average and maximum life span in mice [18,19]. PBN rapidly penetrates to most tissues and suppresses production of ROS by mitochondria and also inhibits gene transduction caused by oxidative insults. Synthetic salen-manganese complexes termed EUK (Eukarion) were developed to mimic activities of the endogenous superoxide dismutase . Tempol, a stable nitroxide free radical, is another superoxide dismutase mimetic.

N-acetyl cysteine is similar to Tempol.

N-acetyl cysteine (NAC) is another pharmacological thiol-containing antioxidant which acts according to several mechanisms. new types of interventions mimicking the effect of caloric restriction or the effect of other stressor while avoiding their negative aspects have been developed. (i.e. 2-deoxy-D-glucose, resveratrol) [54,55], chemical mimetics of heat shock (i.e. curcumin) [56]. Thus, a new concept has been introduced by Lamming: xenohormesis, which means hormesis induced by biochemical compounds . Exposure to low concentrations of various phytochemicals (resveratrol, tannic acid, juglone) induces potent life-prolonging properties in the nematode worm Caenorhabditis elegans, at least in part due to increased resistance to oxidative stress .
Carnitines (carnitine/acetylcarnitine) have recently been demonstrated to be neuroprotective through the activation of hormetic pathways.
There is also one study where scientists found that a chronic dose of acetylcarnitine decreased mortality in animals, showing a prolongevity potential< BR>Ozone, another candidate for the pharmacological anti-aging hormetins, when used at appropriate doses, promotes the generation of reactive oxygen species and a consequent antioxidant response capable of reversing the oxidative stress [37]. Findings of an increased level of antioxidant enzymes during ozone therapy show the hormetic potential of this pharmacological agent
The two end points of the hormesis curve, which are physical inactivity and overtraining, result in increase of both oxidative stress and incidence of age-related disorders [38,69,70], while the regular moderate exercise induces a ROS mediated adaptation which results in enhanced activity of antioxidant and damage repair enzymes, lower levels of oxidative damage and prevent various age-associated loss (e.g. decreased skeletal muscle function, cognition
http://medandlife.ro/medandlife639.html< /STRONG>

Juglone is found in black walnuts, pecans and teak bark
Juglone is so toxic only minute amounts can sicken, sedate, or kill people and animals.

http://www.worldscientific.com/doi/abs/10.1142/s0192415x10007737
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1985Articles/Phyto75n12_1419.pdf


 

L-Theanine extends lifespan of adult Caenorhabditis elegans
http://link.springer.com/article/10.1007/s00394-012-0341-5#page-1

Green and Black Tea contain the highest levels of Theanine
http://www.ehow.com/list_7175223_food-sources-theanine.html

 

< P>Nutraceutical Interventions for Promoting Healthy Aging ,
Supplementation of spermidine prolongs the lifespan of C. elegans and Drosophila by 15% and 30%, respectively . Ginkgo biloba Extract ,Nectarine and Aaci, Rosa damascena, 

Putrescine then forms spermidine and spermine through a reversible metabolic process.  Spermidine content was high in dry soy bean, Sweedish Hard Cheese, green peas, corn, shell fish, and blue cheese. A high content of spermine was found in most of the meat products (like sausages, pork, chicken, and turkey), some vegetables (like pumpkin), and cheese. The polyamines spermine and spermidine protect proteins from structural and functional damage by AGE precursors: a new role for old molecules. Fermented soy bean (Natto) has been reported to have considerable amounts of polyamines. 
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022763/

 

We showed a significant positive association between GDP and the amount of polyamine per 1000 kcal of food. As several basic research studies have shown that polyamines help prolong longevity, it follows that polyamines may have a role in determining the lifespan of humans.
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=3577

Longevity in Mice Is Promoted by Probiotic-Induced Suppression of Colonic Senescence Dependent on Upregulation of Gut Bacterial Polyamine Production
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023652

Our study demonstrated increased longevity in mice following probiotic treatment with LKM512, possibly due to the suppression of chronic low-grade inflammation in the colon induced by higher PA levels. This indicates that ingestion of specific probiotics may be an easy approach for improving intestinal health and increasing lifespan.
 
http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0023652

Polyamines are also called putrescine, spermidine, and spermine
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022763/

 


The lifespan-promoting effect of acetic acid and Reishi polysaccharide
http://www.sciencedirect.com/science/article/pii/S096808960900844X

Foods high in Acetic Acid: Apple Cider Vinegar, Distilled White Vinegar, Pickles and pickled foods, Sourkrout, Wine
http://www1.extension.umn.edu/food/food-safety/preserving/pickling/vinegar-for-pickling/

< P>Effects of bioactive extracts from four edible mushrooms on the lifespan of Drosophila melanogaster . The results showed that 5 mg/ml of L. edodes and A. blazei extracts extended the lifespan of male and female flies by 40.53 and 6.03%, and 32.13 and 2.69%, respectively. An extract from A. auricula extended the lifespan of male flies by 31.41% at 5 mg/ml and female flies by 16.85% at 20 mg/ml. While an extract from G. lucidum (Lingzhi Mushroom ) extended the lifespan of male flies by 42.32% at 80 mg/ml and female flies by 29.24% at 5 mg/ml.
http://www.sciencedirect.com/science/article/pii/S096808960900844X

L. edodes is known as Shittake Mushroom,  A. blazei is also called Almond Mushroom - Scientific Name: Agaricus subrufescens, A. auricula - Scientific Name: Auricularia auricula-judae, G. lucidum is also known as Reishi Mushroom.
http://en.wikipedia.org/wiki/Shiitake

Research data showed acetic acid (vinegar) extended C. elegans lifespan by 25%. Mushroom extracts, including Reishi F-3 polysaccharide (Ganoderma lucidum), mycelium fractions of Chang-Chih (A. camphorata), and lion�s mane (H. erinaceus) were associated with significant increase of lifespan, ranging from 25% to 35%. A synergistic, life-extending effect was found when acetic acid and RF-3 were combined (40%).
http://online.liebertpub.com/doi/pdfplus/10.1089/acm.2013.1501

Foods highest in Fructose:
Applesauce, Honey, Pears, Agave, Apple and Grape Juices, Pomegrants, Pears, Dates, Watermelon,
http://nutritiondata.self.com/foods-000011000000000000000.html


Foods highest in Glucose.
Summer Squash, Molasses, Oranges, Tangerines, Mandarines, Pineapple, Raspberries, Grapefruit, Nectarines, Raw Celery, Lettuce, Raw Asparagus, Raw Carrots, Lime Juice
http://nutritiondata.self.com/foods-000010000000000000000-4.html

Foods highest in Sucrose.
Brown Sugar, Maple Syrup, Canned Beets, Raw Cantaloupe or Watermelons, Clementines, Pineapple, Apricots, Peaches, Pineapple, Tangerines, Carrots, Nectarines, Molasses, Dates, Grapefruit,
http://nutritiondata.self.com/foods-000009000000000000000.html

Foods highest in disaccharides.
Sugar Beets and Sugar Cane


Foods high in Trehalose
Trehalose is available as an extract on amazon.com and is high in the foods honey, wine and mushrooms. It is especially high in foods that have been fermented using brewers yeast

< P>Dietary Supplements May Increase Longevity by Up-Regulation of daf-16/FoxO Gene
http://online.liebertpub.com/doi/pdfplus/10.1089/acm.2013.1501

Niacin-bound chromium increases life span in Zucker Fatty Rats
http://www.ncbi.nlm.nih.gov/pubmed/21930012

Provision of free water in addition to sugar solution is necessary for optimum longevity by female and male flies. Longevity is increased by 2.4�2.6-fold by the two monosaccharides, fructose and glucose, and by 2.6�2.8-fold by the disaccharides, sucrose and trehalose.
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3032.2005.00484.x/abstract

< P>Vitamin d2 enriched mushrooms and fungi for treatment of oxidative stress, alzheimer's disease and associated disease states
http://www.google.com/patents/WO2011035314A2

Foods high in Vitamin D2 - eggs, cod liver oil, sardines

Rhodiola: a promising anti-aging Chinese herb
http://www.ncbi.nlm.nih.gov/pubmed/17990971

Cinnamomi cassiae and Rhodiola rosea extracts significantly decreased on blood glucose, increased levels of reduced glutathione
http://onlinelibrary.wiley.com/doi/10.1002/biof.5520260306/abstract

< P>Adaptogens exert a stress-protective effect by modulation of expression of molecular chaperones
http://www.sciencedirect.com/science/article/pii/S0944711308002250

A fixed combination of three genuine (native) extracts of Eleutherococcus senticocus, Schisandra chinensis and Rhodiola rosea were given to mice which were exercised to exhaustion. Eleutherococcus senticocus is known as Siberian Ginseng, Schisandra chinensis is known as Schisandra berry.
http://www.ncbi.nlm.nih.gov/pubmed/19188053

< P>We mined the information on traditional Indian medicines and identified an iridoid, 10-O-trans-p- Coumaroylcatalpol (OCC), a major ingredient of Premna integrifolia Linn. (syn: Premna serratifolia). OCC forms an important constituent of famous herbal formulation ��Dashmula��, a ten herb formulation, commonly used for its various medicinal properties. OCC extended the mean life span of nematodes, increased their tolerance against chemical induced stress, improved the chemotaxis index and reduced the ROS content
http://www.ingentaconnect.com/content/ben/cnsnddt/2012/00000011/00000008/art00007
Dashamula Formula:
Ingredients: Bilva root (Aegle marmelos), Agnimantha root (Premna integrifolia), Shyonaka root (Oroxylum indicum), Patala root (Stereospermym suaveolens), Kashmari root ( Gmelina arborea), Bruhati root (Solanum indicum), Kantakari root ( Solanum xanthocarpum), Shalaparni root ( Desmodium gangeticum), Prushniparni root ( Uraria picta), Gokshura root (Tribulus terrestris).
http://www.banyanbotanicals.com/prodinfo.asp?number=7552

 

< P>The aim of our studies on plant adaptogens, e.g. ADAPT-232 forte - a fixed combination of standardized extracts of Eleutherococcus senticocus (Rupr. et Maxim) Harms root, Schisandra chinensis (Turzc) Baill., root, Rhodiola rosea L., root resulting in enhanced mental and physical performance and increased of longevity [6].
https://www.thieme-connect.com/ejournals/abstract/10.1055/s-0029-1234262

< P>A research group in Japan explored the potential role of green tea polyphenols and tetrahydrocurcumin in increasing the life spans of animal models. Their studies revealed that the increase in life expectancy beyond 24 months under the conditions of the experiment was 125.9% in tetrahydrocurcumin treated mice , and 72.6% in polyphenols treated animal models, as compared to untreated controls< BR>Tetrahydrocurcuminoids
In a follow-up study, (Kitani, K et al.; 2007) the effect of feeding of the two natural antioxidants,
tetrahydrocurcumin (THC) and green tea polyphenols (PPs) on the survival of male C57BL/6 mice
was examined. Mice that started to receive diets containing THC (0.2%) at the age of 13 months had significantly longer average life spans than control mice (11.7% increase, p < 0.01).
http://highered.mcgraw-hill.com/sites/dl/free/0073370932/812086/san70932_ch01.pdf

Tetrahydrocurcumin is a spice derived from the spice turmeric of which the compound curcumin is high in.
Our findings suggest that the combination of curcumin and catechins (catechins are high in Green Tea) may produce a synergistic colon cancer-preventative effect that would be more potent than each of the compounds alone.
http://www.ncbi.nlm.nih.gov/pubmed/19861145

 

The hydrogenated form of curcumin is Tetrahydrocurcumin, which is an off-white colour and can be added to skin care products to function as an antioxidant and to protect the lipids in the moisturiser from going rancid. Cosmetic chemists report the antioxidant effect of tetrahydrocurcumin to be greater than that of vitamin E.
http://www.drfrances.co.nz/is-natural-best.html

 

< P>Longevity in Mice Is Promoted by Probiotic-Induced Suppression of Colonic Senescence Dependent on Upregulation of Gut Bacterial Polyamine Production
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023652

Ginseng boosts Gut microbiota
http://www.ncbi.nlm.nih.gov/pubmed/23638073
  

< P>Here, I discuss the rationale and recent evidence for probiotic-induced upregulation of intestinal luminal polyamine (PA) production in the extension of lifespan by preventing inflammageing.
http://www.sciencedirect.com/science/article/pii/S0306987711002696

< P>Gut microbiota as a candidate for lifespan extension:
http://link.springer.com/article/10.1007/s10522-011-9352-5

Probiotic properties of lactic acid bacteria isolated from stool samples of longevous people . The results presented here show that L. rhamnosus LV108, L. rhamnosus F, B. brevise R39 and B. infantis R42 are acid and bile tolerant, adhere to the cultured human intestinal Caco-2 cell line, antagonistic activity against potential pathogenic bacteria infection in vitro, and so are potential strains for probiotic use.
http://www.sciencedirect.com/science/article/pii/S1075996408000747

< P>One strain, identified as Lactobacillus rhamnosus CNCM I-3690, protected worms by increasing their viability by 30% and, also, increased average worm lifespan by 20%.< BR>http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0052493

Lactobacillus rhamnosus can be found in yogurt, fermented milk, pasteurized milk, and semi-hard cheeses
http://microbewiki.kenyon.edu/index.php/Lactobacillus_rhamnosus_GG_(ATCC_53103)_and_its_Probiotic_Use

Lactobacillus rhamnosus GG is found in provolone and parmesan cheeses
http://keywen.com/en/LACTOBACILLUS_RHAMNOSUS_GG

 

< P>The findings of this study suggest that the nematodes fed the respiratory deficient E. coli diet live longer because the delay in bacterial colonization of the gut subjects the worms to less stress compared to worms fed the OP50 E. coli diet
http://www.biomedcentral.com/1471-2180/12/300/

Bacterial Nitric Oxide Extends the Lifespan of C. elegans
http://www.sciencedirect.com/science/article/pii/S0092867413000147

The nitrates in beet juice react with bacteria in your saliva and stomach to produce nitric oxide in your body.
http://www.purecleanpowder.com/pages/beet-science

< P>After 5 years, cats fed the diet with the antioxidants vitamin E and �-carotene, dried chicory root and a blend of n-3 and n-6 fatty acids lived significantly longer than cats fed the control diet
http://www.jarvm.com/articles/Vol5Iss3/Cupp%20133-149.pdf

Foods Highest in VItamin E:
Wheat Germ, Hazelnut Oil, Peanut Butter, Sunflower Oil and Sunflower Seeds, Alsmonds and Almond Oil
http://nutritiondata.self.com/foods-000103000000000000000-w.html?categories=3,10,11,14,0,19,22,15

Inulin comes from Chicory Root. FOods high in Inulin include: chicory, garlic, and leek,
http://en.wikipedia.org/wiki/Inulin

Foods highest in Beta Carotene, Lettuce, Kale, Spinach, Dandelion Geens, Carrots, Mustard Greens,
http://nutritiondata.self.com/foods-000135000000000000000.html

 

 

We report that metformin increases lifespan by altering microbial folate and methionine metabolism
http://www.sciencedirect.com/science/article/pii/S0092867413002675

< P>Methanolic extracts of C. crispus (CCME) enhanced C. elegans tolerance to juglone-induced oxidative stress and increased life span; however water extracts did not show such effects
http://www.sciencedirect.com/science/article/pii/S1756464613001060

C. crispus commonly called Irish moss or carrageen moss � is a species of red algae which grows abundantly along the rocky parts of the Atlantic coast of Europe and North America.

< P>Lactoferrin and brain health and protection in adults
http://www.freepatentsonline.com/EP2251032.html

Season of Birth and Human Longevity
http://online.liebertpub.com/doi/abs/10.1089/rej.1.1999.2.365

Lifespan of Africanized honey bees fed with various proteic supplements.
The results demonstrated that the supplementation with a mixture of linseed oil plus palm oil, and isolated soy protein plus brewer's yeast is recommended to increase the royal jelly production on a commercial scale.
http://www.scielo.br/scielo.php?pid=S1807-86722013000200009&script=sci_arttext

< P>The optimal conditions for a long life (of rats) proved to be thin bodies, exercise and a low protein diet with the protein supplied by liver.� Slonaker's (1935) experiments also indicated that the life span of both virgin and bachelor rats was shortest in the group which were fed higher (26%) protein diet
http://www.ingentaconnect.com/content/esa/aesa/1953/00000046/00000004/art00015

< P>Effect of caloric restriction on life span of the housefly, Musca domestica
Sucrose was found to promote a longer life span than diets additionally containing proteins and lipids
http://www.ncbi.nlm.nih.gov/pubmed/15319362

Foods highset in sucrose: Maple Syrup, Beets, Coconut Cream, Cantaloupe, Clemintines, Pineapple, Apricots, Pecahes, Tangerines, Nectarines,
http://nutritiondata.self.com/foods-000009000000000000000.html



Cinnamomum cassia bark in two herbal formulas increases life span in Caenorhabditis elegans via insulin signaling and stress response pathways
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0009339
 
Epigallocatechin gallate (EGCG), a main active ingredient of green tea, is believed to be
beneficial in association with anticarcinogenesis, antiobesity, and blood pressure reduction.< BR>Here we report that EGCG extended Caenorhabditis elegans longevity
http://www.sciencedirect.com/science/article/pii/S089158490800662X

Juzen-taiho-to (TJ-48), a mixture of extracts from 10 medicinal herbs, has been used traditionally to treat patients with anemia, anorexia, or fatigue. Young (3-month-old) and aged mice (18-month-old) were fed a diet containing Japanese herbal medicine (TJ-41 or TJ-48) for 5 months, and the effect of the herbal medicines were examined in terms of levels of monoamines and their metabolites in the brain of young and aged mice. In the aged mice, the levels of norepinepherine, serotonin and their metabolites in the brain were decreased in the cortex, hippocampus and hypothalamus
http://link.springer.com/article/10.1007/s11064-004-9688-1
http://www.ncbi.nlm.nih.gov/pubmed/18837771

Juzen-taiho-to Formula
 
Ogi (Astragali Radix) 3g
 Keihi (Cinnamomi Cortex), 3g
 Bukuryo (Sclerotium Poriae Cocos), 3g
 Kanzo (Glycyrrhizae Radix), 2g
 Ninjin (Ginseng Radix), 2g
 Byakujutsu (Atractylodis macrocephalae Rhizoma) 3g
 

Jukujio (Rehmanniae Radix preparata), 3g
 Shakuyaku (Paeoniae Radix) 3g
 Senkyu (Chuanxiong Rhizoma) 3g
 Toki (Angelicae sinensis Radix) 3g

The common yeast, S. cerevisiae, is often used in laboratory experiments as a model for understanding the biology of higher organisms because of the great similarity of basic life processes.2 When scientists added the unique apple polyphenol phloridzin to yeast cells in culture, they found that treated cultures lived an average of 2.3 generations longer than control cultures. One polyphenol, phloridzin, which is heavily concentrated in the skin of the apple, also potently suppresses several processes leading to glycation, another major contributor to aging
http://www.lifeextensionretail.com/MagazineArticleDetail.aspx?article=119072

phloridzin is found in high levels in: paradise apple species, Prune Juice, Apple Cider, Oregano
 
http://www.phenol-explorer.eu/contents/polyphenol/109

< P>Beer Yeast increased lifespan by 41%. A mixture of equal parts of linseed oil and palm oil increased longevity.
http://www.jas.org.pl/jas_54_2_2010_4.pdf

is trans-palmitoleic acid the longevity secret of Japanese?
 
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=33132

Palmitoleic acid Content of Foods
 Macadamia oil (Macadamia integrifolia) and sea buckthorn oil
 (Hippophae rhamnoides) are botanical sources with high concentrations,
 containing 17%[2] and 40%[3] of palmitoleic acid, respectively.
http://en.wikipedia.org/wiki/Palmitoleic_acid

Autum born mice suffered ligher mortality than spring. < BR>http://www.jstor.org/discover/10.2307/1382107?uid=3739464&uid=2&uid=3737720&uid=4&sid=21102667297207

< P>Isolation and Caenorhabditis elegans Lifespan Assay of Flavonoids from Onion
Quercetin showed the highest antioxidative activity, whereas Q3M showed the strongest antiaging activity among these flavonoids, which might be related to its high hydrophilicity.
http://pubs.acs.org/doi/abs/10.1021/jf104798n

A polyphenol enriched cocoa extract with 28% of procyanidins has been obtained. ► The extract was efficient as free radical scavenger< BR>http://www.sciencedirect.com/science/article/pii/S0278691511006508

These findings suggest that cranberry is of particular benefit to β-cell function in normal aging rats
http://biomedgerontology.oxfordjournals.org/content/66A/11/1139.short

Rapamycin fed late in life extends lifespan in genetically heterogeneous mice
http://www.nature.com/nature/journal/v460/n7253/full/nature08221.html

Rapamycin Extends Maximal Lifespan in Cancer-Prone Mice< BR>http://www.sciencedirect.com/science/article/pii/S0002944010600065
 
Low concentrations of rapamycin extend yeast chronological life span (CLS). < BR>
https://www.landesbioscience.com/journals/autophagy/article/8824/

N−3 polyunsaturated fatty acids impair lifespan but have no role for metabolism
http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2006.00257.x/full

< P>The addition of α-tocopherol (vitamin E) to the culture medium at a concentration of 10−4 was found to significantly extend the average lifespan of the rotifer Philodina.
http://www.sciencedirect.com/science/article/pii/053155658090039X
 
Bats fed purely vegetarian diets were stunted in growth.
Rats fed an omnivorous diet lived significantly longer than
rats fed a vegetarian diet. Moreover, the life span was more
uniformly prolonged by the intermittent fasting of rats fed
 an omnivorous diet than in rats fed a vegetarian diet
http://jn.nutrition.org/content/34/1/81.full.pdf

In conclusion, we demonstrated that arginine methylation acts as an �antiaging� modification against the IIS pathway in C. elegans
http://www.sciencedirect.com/science/article/pii/S1550413111001410

Protein methionine content and MDA-lysine adducts are inversely related to maximum life span in the heart of mammals
http://www.sciencedirect.com/science/article/pii/S004763740500117X

Histone H4 lysine 16 acetylation regulates cellular lifespan
http://www.nature.com/nature/journal/v459/n7248/abs/nature08085.html

Low Moisture Content Limits to Relations Between Seed Longevity and Moisture
http://aob.oxfordjournals.org/content/65/5/493.short

The optimal conditions for a long life (of rats) proved to be thin bodies, exercise and a low protein diet with the protein supplied by liver.� Slonaker's (1935) experiments also indicated that the life span of both virgin and bachelor rats was shortest in the group which were fed higher (26%) protein diet.
http://www.youtube.com/watch?v=2MEBxRM7Ofs< /FONT>

The most widely used compound in this series, α-phenyl-tert-butyl-nitrone (PBN), has been shown to extend life span in three published studies, i.e. two mouse models and one rat model. Significant prolongation of life span was noted in all three studies. We report the summary of a recent study with a novel nitrone, CPI-1429, which demonstrated the ability to extend life span even though administration of the compound was begun in older animals.
http://www.sciencedirect.com/science/article/pii/S0047637401003852< /FONT>

Nitrones as Neuroprotectants and Antiaging Drugs
http://www.ncbi.nlm.nih.gov/pubmed/11976206< /FONT>

A Spin Trap, N-tert-Butyl-α-phenylnitrone Extends the Life Span of Mice
https://www.jstage.jst.go.jp/article/bbb/62/4/62_4_792/_article

Females and males of M. mediator fed with 1 M sucrose solution survived longer than controls fed with water (5.7- and 3.7-fold longer, respectively). When provided with sucrose, glucose or fructose solutions, the parasitoid generated 3.6- to 3.7-fold more offspring than controls, and 60�75% of these progenies were produced during the first 5 days. When separately given fructose, sucrose or glucose, this wasp accumulated fructose and total sugar at the highest level, which means a high sugar levels might lead to prolonging longevity and more offspring in M. mediator
http://www.sciencedirect.com/science/article/pii/S1049964409002308< /FONT>

As expected, sucrose-fed individuals lived longer than did starved individuals
http://journals.tubitak.gov.tr/agriculture/issues/tar-10-34-4/tar-34-4-8-0904-21.pdf< /FONT>

Comparing the effects of five naturally occurring monosaccharide and oligosaccharide sugars on longevity and carbohydrate nutrient levels of a parasitic phorid fly, Pseudacteon tricuspis
http://pubget.com/paper/pgtmp_95da880e6370597ff5f220561a49af44/comparing-the-effects-of-five-naturally-occurring-monosaccharide-and-oligosaccharide-sugars-on-longevity-and-carbohydrate-nutrient-levels-of-a-parasitic-phorid-fly-i-pseudacteon-tricuspis-i< /FONT>

The optimal lifetime was achieved by insects fed with 50 and 70% (w/v) of sugar solution for males and 70% (w/v) for females.< BR>http://www.sciencedirect.com/science/article/pii/S1049964404001422

The beneficial effects of theanine in tea are reported on many aspects. Here we report that theanine can extend nematode Caenorhabditis elegans lifespan under stress. Theanine (100 mg/mL) significantly increased the mean longevity of C. elegans by 12.8% and 21.3% under heat stress and oxidative stress, respectively.
http://www.sciencedirect.com/science/article/pii/S1756464612000539

L-Theanine
L-theanine is found mainly in tea (eg, green tea and black tea ), and to a less extent in mushrooms. L-theanine is also available in its purified form as an oral dietary supplement. 1
http://www.med.nyu.edu/content?ChunkIID=653856

 

Fruit Fly Experiments that Resulted in Longer Life Spans

Alcohol dehydrogenase is also involved in the toxicity of other types of alcohol: For instance, it oxidizes methanol to produce formaldehyde and ethylene glycol to ultimately yield glycolic and oxalic acidsIn early 1960 it was discovered in fruit flies of the genus Drosophila These are raw figures from a researcher testing which compounds extend the life of fruit flies. As we can see below approaching day 60, almost 10 fruit files were still alive during the best experiment.
Supple-
ment ------ % of Survival 13 18 27 33 38 43 48 60 69 75 81 86
control one 86 79 57 36 18 14 7 0 - - - -
control two 86 80 69 40 34 23 14 3 0 - - -
maltitol 1/2 tsp. 97 92 81 69 58 44 36 19 3 0 - -
maltitol 1 tsp. 86 86 72 62 48 41 28 17 10 3 0 -
maltitol 2 tsp. 100 100 90 74 63 58 42 37 0 - - -
maltitol 3 tsp. 96 96 85 78 44 30 30 11 4 4 0 �
1,3-propanediol 1/8 tsp. 50 32 29 21 15 12 9 3 0 - - -
1,3-propanediol 1/2 tsp. 85 82 67 52 39 33 21 9 0 - - -
sucrose 2 tsp. 97 97 97 93 77 67 50 23 17 7 3 0

triethylene glycol 1/8 tsp. 92 89 73 50 35 19 12 0 < /FONT>

triethylene glycol 1/2 tsp. 91 78 44 26 22 9 4 0 - - - -

PEG200 1/8 tsp. 100 86 68 50 32 18 14 0 ( PEG 200 IS USED IN THE ZERO POINT ENERGY BATTERY)

PEG200 1/2 tsp. 62 42 19 8 0 - - - - - - -

Research Source:
http://www.disease-treatment.com/showthread.php?t=62254


So let us analyze these substances further:< /FONT>

1st Substance: Maltitol. Maltitol has been proven to heal cavities and is used as a natural sweetener.< /FONT>

2nd Substance: Propanediol. Vanilla flavor, of which the longevity substance Vanillin comes from, is made with glycerine and propylene glycol.  Propylene glycol is also known as 1,2-propanediol. Its special qualities is that it soaks up water like a sponge. Much like the vascular plant system, which has the ability to retain moisture over long periods of time.  < /FONT>

Definition of Propylene Glycol (also called PG and is very similar in structure to ethylene).  A form of mineral oil, an alcohol produced by fermentation of yeast and carbohydrates.  PG comes in several grades and is used for a variety of uses. Industrial grade PG is used as an active ingredient in antifreeze, airplane de-icers; polyurethane cushions; engine coolants, paints, enamels and varnishes; and in many products as a solvent or surfactant.  Recently PG was added to anti-freeze to replace Ethylene Glycol, because dogs often lap up puddles of anti-freeze.

Food grade Propylene glycol is available commercially on amazon.com and other reliable internet merchant sites.  Biologically-derived propanediol is also used in bakery goods
Sources:< BR>
http://www.google.com/patents/US20070275139

http://www.v2cigs.com/blog/2012/01/what-is-propylene-glycol/ < /FONT>

So it appears that if food grade propanediol is used in small amounts it is okay, however larger amounts may be problematic.

The 3rd substance, Glycol, a component of Ethylene, which is covered in more detail in my other book titled: Living Healthy Beyond 120, A Centurion's Plan for Longevity.

Another substance that retains moisture is ethanol.< /FONT>

�Small amounts of food grade ethanol more than double the life span of the Caenorhabditis elegans, a worm used frequently as a model in aging studies.�
http://newsroom.ucla.edu/portal/ucla/tiny-amounts-of-ethanol-dramatically-221986.aspx< /FONT>

So we can see a connection between these substances in that they attract and retain air moisture, also known as humectants.< /FONT>

Humectants attract and retain moisture in the air by absorption.  This than allows the water to be used by other substances. Unlike a desiccant which also attracts moisture, but adsorbs it instead of absorbing. For example it will condense the water onto a surface as a layer of film, much like dew collects on the grass. As a food additive, humectants keep foods moist. One example is Potato flour, which attracts moisture and keeps breads moist for longer.

Definition of adsorb: �Molecules of liquid, gas or solute.  These hold as a thin film on the outside surface or on an internal surface.�

Many longevity foods that are Humectants include: xylitol and maltitol, sugar polyols, lactic acid, aloe vera gel, honey, urea and glycerin. Lithium Chloride is one of the best hemectants, but is toxic. < /FONT>

Foods high in Lithium include: Eggs and Milk contain the highest levels.  Other sources include: Plant ash (shale), Seaweed, sugarcane, tobacco,  Lemons, potatoes, and natural brines. < /FONT>

Source: U Schafer, �Essentiality and Toxicity of Lithium�, Journal of Trace and Microprobe Techniques, 15(3), 341- 349, 1997< /FONT>

Further Reading:
http://en.wikipedia.org/wiki/Humectant

As we covered earlier in this article, these Humectants have a tendency to cling to the outer walls of blood vessels as well as the vasular vessel nutrient distribution in plants.< /FONT>

Enzo Nisoli of Milan University and his colleagues report in the October, 2010 issue of the journal Cell Metabolism that branched-chain amino acids, which include leucine, isoleucine and valine, extended the average life span of mice when administered in their drinking water.
http://www.lef.org/newsletter/2010/1005_Branched-Chain-Amino-Acids-Extend-Life-Span-in-Animal-Model.htm

Activate Your Longevity Genes: Five Natural Compounds Simulate Caloric Restriction
Resveratrol, pterostilbene, Quercetin, Grape seed extract and Black tea extract.    
http://www.lef.org/magazine/mag2010/feb2010_Activate-Your-Longevity-Genes-Calorie-Restriction_01.htm

Fisetin from fruits and vegetables protects the aging brain and helps fight cancer
Learn more:
http://www.naturalnews.com/034545_fisetin_fruits_cancer.html#ixzz2g2vSAHBl

Fisetin is found in species of the genus Rhus, which contains the sumacs. Along with myricetin, fisetin provides the color of the traditional yellow dye young fustic, which was extracted from the Eurasian smoketree (Rhus cotinus). fruits and vegetables also contain fisetin, strawberries[15][19] apples,[19] and grapes.It is also found in Monocotyledons such as onions.[19] It is also present in Pinopyta species such as the yellow cypress (Callitropsis nootkatensis).
 Interesting how the color yellow pops up frequently here.
http://en.wikipedia.org/wiki/Fisetin

Author's Note: You can add synergy with these extracts by taking foods that are high in them. A good example is Grapefruit, which is high in vitamin C, take with a Vitamin C tablet.  Therefore taking apples and a small amount of qucertin extract will also create major synergy.

 

Resveratrol at 400 μM extended lifespan of females fed a high-fat diet.
http://link.springer.com/article/10.1007/s11357-011-9332-3#page-1

Black tea theaflavins extend the lifespan of fruit flies
http://www.sciencedirect.com/science/article/pii/S0531556509001922

Our results illustrate that a moderate supplementation of cocoa under normoxia increases the average life span, whereas, at higher concentrations, average life span is normal. Finally, we illustrate that cocoa also acts as a metal chelator in the presence of excess heavy metals
http://www.nrjournal.com/article/S0271-5317(08)00092-4/abstract

Results demonstrated that 0.2 % sesamin in diet prolonged the mean lifespan of OR wild fruit flies by 12 %
http://link.springer.com/article/10.1007/s10522-012-9413-4#page-1

 Sesamin is a lignan derived from sesame seeds (Sesamum indicum) that appears to inhibit vitamin E metabolism, which causes a relative increase in circulating levels of γ-tocopherol and γ-tocotrienol; it shows most promise in augmenting the efficacy of vitamin E supplements.

Black rice extract extends the lifespan of fruit flies by 14%.
http://pubs.rsc.org/en/content/articlelanding/2012/fo/c2fo30135k#!divAbstract

These results suggest that kimchi, especially OptR kimchi, may delay the aging process by regulation of inflammatory process
http://link.springer.com/article/10.1007/s10068-011-0091-9#page-1

The present study demonstrated that Kangenkaryu prevented oxidative damage and mitochondrial dysfunction with ageing.
http://onlinelibrary.wiley.com/doi/10.1211/jpp.58.12.0005/abstract

Kangenkaryu is a chinese prescription drug

β-Carotene (1 μM), lutein (1 μM) and astaxanthin (10 nM) protect against UVA light-induced oxidative stress in vitro with astaxanthin exhibiting superior protective properties.
http://www.jdsjournal.com/article/S0923-1811(97)00058-3/abstract

astaxanthin  is available as a supplemnet on amazon.comazon.com

Alpha-carotene, beta-carotene, and beta-cryptoxanthin are provitamin A carotenoids, meaning they can be converted by the body to retinol (vitamin A).
Research has suggested a minimum of 6-10 mg per day of lutein is necessary to realize lutein's health benefits.
Lutein synergizes with zeaxanthin and Astaxanthin

Bama County is famous for longevity. The ratio was 3.52 centenarians per ten thousand people, seemingly the highest found anywhere in the world.  the secret to longevity of the people of Bama is attributable, at least in part, to the daily consumption by both villagers and their herds of a plant they call shilianhua, meaning in English �rock lotus� or �stone lotus.� Another Chinese name for the plant is He Hua Zhang.
http://www.totalhealthmagazine.com/articles/anti-aging/uncovering-the-longevity-secrets-of-the-rock-lotus.html

Antioxidants found specifically in freeze-dried acai extract have been shown to enter human cells in a fully functional form and effectively neutralize free radicals at very low doses
http://www.lef.org/magazine/mag2010/jun2010_the-science-behind-acai_01.htm

�Antioxidants found specifically in freeze-dried acai extract have been shown to enter human cells in a fully functional form and effectively neutralize free radicals at very low doses.13
� Similarly, freeze-dried a�a� extract has demonstrated unrivalled scavenging capacity against two of the most damaging reactive oxygen species, the superoxide and peroxyl radicals.14
 �A randomized, double-blind, placebo-controlled study on a standardized elderberry product found that it reduced the duration of the flu symptoms to just 3-4 days.84 The study also showed it to be effective against 10 different strains of influenza.
 �
13. Schauss AG, Wu X, Prior RL, Ou B, Huang D, Owens J, Agarwal A, Jensen GS, Hart AN, Shanbrom E. Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae mart. (acai). J Agric Food Chem. 2006 Nov 1;54(22):8604-10.
 
14. Schauss AG, Wu X, Prior RL, Ou B, Huang D, Owens J, Agarwal A, Jensen GS, Hart AN, Shanbrom E. Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae mart. (acai). J Agric Food Chem. 2006 Nov 1;54(22):8604-10.
 
http://www.lef.org/magazine/mag2010/jun2010_The-Science-Behind-Acai_01.htm  
In SODn108 mutant flies, the supplementation of HP (10 or 20 mg/mL) significantly extended their lifespan and ameliorated the age-related decline in locomotor function. Further studies suggested that HP may play a role as a complement to the defective endogenous antioxidant system to exert such lifespan elongation effects. These results, taken together, strongly support the antiaging properties of HP and its therapeutic rather than preventive potential against aging-related diseases.
 (Authors Note: Therefore Superoxide Dismutase and Astaxanthin synergize with each other). Astaxanthin is produced by the microalgae Haematococcus pluvialis
http://pubs.acs.org/doi/abs/10.1021/jf402224w

HP stands for microalga Haematococcus pluvialis (HP), natural producer of astaxanthin (AX), which is a potent antioxidant with broad health benefits

Fruit Fly Experiment Results:
Nicotinamide, Chlorophyll and Sage extend life the longest.
Other chemicals include: Forskolin, NADH, Pyroglutaminc and Xanthophyll.
Another group included: Activated charcoal, Celery seed, Citrus bioflavonoids, Malt.
Purple yam and Sage had the highest.
http://www.quantium.plus.com/lr/lr60.htm

Extension of Human Cell Lifespan by Nicotinamide
http://www.jbc.org/content/282/15/10841.short

Nicotinamide  is available as a supplemnet on amazon.comazon.com

 

Life-span experiments revealed that the lower concentrations of orange juice, hesperidin, and limonene exerted a positive influence on the life span of Drosophila. Finally all substances showed cytotoxic activity, with hesperidin being least active
http://www.tandfonline.com/doi/abs/10.1080/15287394.2011.582306#.UksLWC3n-Uk

Here, we report that administration of spermidine, a natural polyamine whose intracellular concentration declines during human ageing, markedly extended the lifespan of yeast, flies and worms, and human immune cells. In addition, spermidine administration potently inhibited oxidative stress in ageing mice.
http://www.nature.com/ncb/journal/v11/n11/abs/ncb1975.html


High concentrations of the dopaminergic drug levodopa (L-dopa, L-3,4-dihydroxphenylalanine) administered to mice in their diet affected fertility to a moderate degree and prolonged the mean life-span by a maximum of 50 percent
http://www.ncbi.nlm.nih.gov/pubmed/850799
 

Herbal extracts containing L-DOPA are available. The most common plant source of L-DOPA marketed in this manner is Mucuna pruriens (velvet bean).[4][dead link] L-DOPA is also present in broad beans.[5] The important amino acid called tyrosine is a precursor to L-DOPA and is also available over the counter.
http://en.wikipedia.org/wiki/L-DOPA

------------------------------------------------------------------------------------------------------------------

Rough Analysis Summary of Doug Skreckys Reserach into Fruit Fly Longevity using Extracts, Raw Foods and other life extending substances. Only the foods used that created the longest lived animals are listed

hawthorn 4000 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16057

ginkgo 20 mg
green tea polys 300 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16055

whey protein 4 tsp
elderberry 200%
apple � month april � major longevity
whey protein 4 tsp
In the present experiment an average
longevity of about 40 days for the controls was doubled to about 80 days
with Sun Rype apple juice. Maximum lifespan was also dramatically
increased by 47% from 73 days for the controls, to 107 days for the apple
juice. This breaks my previous record for maximum longevity, which was set
in Run #7 at 88 days.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16020


I tried elderberry tea
this run. However this Nectar consists of a mixture of apple,
elderberry, boysenberry, plum and grape juices
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15676


in comparison to fly longevities reported in medical
journals, a 50 day average lifespan for my control flies is quite
respectible.
threonine 3.4 gm
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15411

lemon
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15213
coconut juice � significant lifespan � November is season
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15038

elderberry nectar 100% - signifncant lifespan
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15038

grape � red � significant
blueberry (canned)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=14632

leucoselect 100 mg +resveratrol 50 mg (additional copper may boost results) Ratio is 1 part leucoselect to 10 parts reservatol or more reservatol than leusselect
rooibos � signifncant anti aging (additional copper may boost results � such as sesame seeds)
synephrine 15 mg
Synephrine, although already known as a synthetic organic compound, was first isolated as a natural product from the leaves of various Citrus trees, and its presence noted in different Citrus juices, by Stewart and co-workers in the early 1960s.[2][3] A survey of the distribution of synephrine amongst the higher plants was published in 1970 by Wheaton and Stewart.[4] It has subsequently been detected in Evodia[5] and Zanthoxylum species,[6] all plants of the family Rutaceae.
Trace levels (0.003%) of synephrine have also been detected in the dried leaves of Pogostemon cablin (patchouli, Lamiaceae).[7] It is also found in certain cactus species of the genera Coryphantha and Dolichothele.[8]
However, this compound is found predominantly in a number of Citrus species, including "bitter" and "sweet" orange varieties.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=14411

50% rooibos tea � signifncant
+leucoselect 200 mg + rooibos tea
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13668

It was shown previously that epithalamin delays age-related changes in
reproductive and immune systems and increases the life span of mice and rats.
Melatonin and epithalamin was added to the nutrition medium and extended lifespan
Thus, it was shown that exposure to epithalamin significantly increases the mean life span and MLS of female D.melanogaster and slowed down their aging rate by 2.12 times
http://www.ncbi.nlm.nih.gov/pubmed/9226628

Mortality rate was decreased by 52% in D. melanogaster, by 52% in rats, by 27% in C3H/Sn mice. It is suggested that antioxidative properties of epithalamin lead to increased lifespan of three different animal species.
http://www.ncbi.nlm.nih.gov/pubmed/9701766

These results not only provide a direct linkage between free radicals/oxidative damage and cognitive performance in old age, but also suggest that synthetic brain antioxidants could be developed to treat or prevent age-associated cognitive impairment and Alzheimer's disease.
http://www.ncbi.nlm.nih.gov/pubmed/8852588



wine +leucoselect 200 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13212

artichoke extract 250mg (possible prevention of erosion of vitamins) � extreme longevity
leucoselect 100 mg
coriander 1/16 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13212

grapefruit, red - signifncant
black cherry- signifncant
passion fruit- signifncant
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=12680

Discovery breakthrough: All of these foods that boosted longevity occur in season during early winter and wintertime
or eating them in the early morning when temperatures are cooler.

Cold showers have been proven to boost the immune system
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211456/



So the ones that extend life in the summer, are summer seasonal foods
Temperature Life Span
52 F 152 days
64 130
68 81
70 86
77 62
81 42
86 25
86 20
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8497
It is possible that during this time of year, eating these foods has a lengenting effect on lifespan. Early winter is also the time of year, people born in September live longest
In celebration of my 100'th fly experiment, I've decided to retest all
the supplements in duplicate, that produced at least one fly that reached
over 105 days of age in the first 99 experiments. These are always from
experiments conducted during the winter, when indoor temperatures are
lower. The supplements are as follows:
Kambiz pomegranate paste (124 days - run# 87),
Knudsen elderberry nectar [freeze concentrated] (118 days - run# 36)
Sun-Rype apple juice + vit C (107 days -run#36)
Huaqi hawthorn juice (106 days - run# 84)
Taisun Enterprises starfruit juice (106 days - run# 83)
Nu'O'C Bi Dao white gourd juice (106 days - run# 83)

hawthorn 4000 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16057

ginkgo 20 mg
green tea polys 300 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16055

whey protein 4 tsp
elderberry 200%
apple � month april � major longevity
whey protein 4 tsp
In the present experiment an average
longevity of about 40 days for the controls was doubled to about 80 days
with Sun Rype apple juice. Maximum lifespan was also dramatically
increased by 47% from 73 days for the controls, to 107 days for the apple
juice. This breaks my previous record for maximum longevity, which was set
in Run #7 at 88 days.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16020

I tried elderberry tea
this run. However this Nectar consists of a mixture of apple,
elderberry, boysenberry, plum and grape juices
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15676


in comparison to fly longevities reported in medical
journals, a 50 day average lifespan for my control flies is quite
respectible.
threonine 3.4 gm
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15411

lemon
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15213

coconut juice � significant lifespan � November is season
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15038

Peanut is one of the potent natural sources of resveratrol. In this study, germination of peanut kernels to enhance resveratrol biosynthesis and preparation of sprouts as a functional vegetable was conducted.  resveratrol contents increased significantly from the range of 2.3 to 4.5 microg/g up to the range of 11.7 to 25.7 mug/g depending upon peanut cultivar after a maximum of 9 days. In comparison with the sprout components, resveratrol contents were highest in the cotyledons ( the embryo within the seed of a plant), slightly lower in the roots, and not detected in the stems
http://www.ncbi.nlm.nih.gov/pubmed/15656656

elderberry nectar 100% - signifncant lifespan
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=15038

grape � red � significant
blueberry (canned)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=14632

leucoselect 100 mg +resveratrol 50 mg (additional copper may boost results) Ratio is 1 part leucoselect to 10 parts reservatol or more reservatol than leusselect
rooibos � signifncant anti aging (additional copper may boost results � such as sesame seeds)
synephrine 15 mg
Synephrine, although already known as a synthetic organic compound, was first isolated as a natural product from the leaves of various Citrus trees, and its presence noted in different Citrus juices, by Stewart and co-workers in the early 1960s.[2][3] A survey of the distribution of synephrine amongst the higher plants was published in 1970 by Wheaton and Stewart.[4] It has subsequently been detected in Evodia[5] and Zanthoxylum species,[6] all plants of the family Rutaceae.
Trace levels (0.003%) of synephrine have also been detected in the dried leaves of Pogostemon cablin (patchouli, Lamiaceae).[7] It is also found in certain cactus species of the genera Coryphantha and Dolichothele.[8]
However, this compound is found predominantly in a number of Citrus species, including "bitter" and "sweet" orange varieties.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=14411

50% rooibos tea � signifncant
+leucoselect 200 mg + rooibos tea
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13668

It was shown previously that epithalamin delays age-related changes in
reproductive and immune systems and increases the life span of mice and rats.
Melatonin and epithalamin was added to the nutrition medium and extended lifespan
Thus, it was shown that exposure to epithalamin significantly increases the mean life span and MLS of female D.melanogaster and slowed down their aging rate by 2.12 times
http://www.ncbi.nlm.nih.gov/pubmed/9226628

wine +leucoselect 200 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13212

artichoke extract 250mg (possible prevention of erosion of vitamins) � extreme longevity
leucoselect 100 mg
coriander 1/16 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=13212

grapefruit, red - signifncant
black cherry- signifncant
passion fruit- signifncant
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=12680

Grapefruit juice increases the
absorption of many drugs, but also increases the risk of kidney
stones.
Mahlab
Marjoram 4X
Royal Jelly
Royal Jelly 4X
Ephedrine 4X
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=10183

Orris root
Savory
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=9938

It looks like aged garlic powder, and the lower dosage of chili have a
mild pro-longevity effect. Both the lower doses of cardamon, and
corriander might also be slightly beneficial before tissue levels
accumulate to a toxic level.
aged garlic 4X
cardamon
chili
coriander
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=9746

hawthorn 4X - extreme longevity
on 4X/cinnamon - extreme longevity
on 4X/paprika/chitosan 4X - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=9427

chitosan 4X - extreme longevity
chromium picolinate 4X - extreme longevity
paprika and spinach
sage 4X - extreme longevity
sumac 4X
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8980

onion 4X - extreme longevity
sumac 4X- extreme longevity
sage 4X- extreme longevity
chitosan 4X- extreme longevity
cinnamon- extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8702

Since the lower dosage of a supplement almost always results in a
greater life span, I am decreasing the dosages I use in the sixth run.Although flies live longer when live
yeast is added to their food, this result was obtained under highly
sterile laboratory conditions.
l-carnosine - lower dose yielded best results- extreme longevity
activated charcoal 4X
l-proline
proline synergizes with glycine
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8597

paprika
purple yam
salt
malt- extreme longevity
paba
l-proline - extreme longevity
l-proline 4X
activated charcoal 4X- extreme longevity
sucrose - extreme longevity
sorbitol
l-carnosine 2X
l-threonine
malt extract and beer yeast are used to make beer
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8497

Activated charcoal - extreme longevity
Anise did well in this run, because the experiment was run in summmer. Ansie is in season during summer
Kelp - extreme longevity
Malt - extreme longevity
Paprika - extreme longevity
Sage
Spinach
Para-aminobenzoic acid (also called paba). Paba is found in spinach and mushrooms - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8461

Anise did well in this run, because the experiment was run in summmer. Ansie is in season during summer
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8428

Rosehip
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8372

activated charcoal
Systems Affected:
Digestion, intestines, and stomach
The Ultimate Heavy Metal Detox
Dean Ornish�s study took 30 men and put them on the typical Ornish program that he effectively employs to reverse heart disease:
After the three month program, blood levels of the participants� enzyme telomerase were 29% higher than when they began, and 24 out of 30 men showed significant increases in their telomerase levels.
http://www.garmaonhealth.com/diet/three-months-longer-life

Taking Niacin than exercising boosts HGH
The growth hormone response to repeated bouts of sprint exercise with and without suppression of lipolysis in men.
In conclusion, suppressing lipolysis resulted in a significantly greater GH response to the second of two sprints, suggesting a potential role for serum FFA in negative feedback control of the GH response to repeated exercise.
http://www.ncbi.nlm.nih.gov/pubmed/18187617

Amchoor - extreme longevity
Melatonin - extreme longevity
melatonin synergises with theanine
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8358

Activated carbon has extended the remaining life span of old rodents by
an impressive 47.3%.
Melatonin 2X
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8358

Unlike omnivores such as mice and men, flies cannot tolerate excess fats
in their diet. Replacing the glucose in the food by various carbohydrates
and fats alters average life span as follows: (4)

Energy Source Life Span
glucose 42.6 days
fructose 44.3
glycerol 43.3
lard 19.5
palmitic acid 17.5
dripping 6.9
butter 7.1
margarine 4.8

Chlorophyll - extreme longevity
Nicotinamide
Basil
Cumin
Green Tea
Nutmeg
Leucoanthocyanins
Sage - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8293

Celery seed
Comfrey
Dill seed
Silica
Tumerin
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8293

all these below - extreme longevity
Nicotinamide
Basil
Chlorophyll
Fenugreek
Nutmeg
Rosemary
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8132

Forskolin - extreme longevity
Xanthophyll - extreme longevity
NADH
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8082

Coenzyme Q10
ALC/COQ10
Chlorophyll
Green Tea
Sage
Thyme
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=8007

Glutamine - extreme longevity
Lycopene - extreme longevity
Pregnenolone - extreme longevity

shen chu cha 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21192

ganmao qingre ghongi 1/2 tsp
loosestrife 1/2 tsp - extreme longevity
ganmao qingre ghongi 1/2 tsp
honey suckle 1/2 tsp
dealcoholized Loxon white wine
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21106

lipoic 200 mg significant anti aging
carnitine + lipoic 100 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20830

ALC 125 mg + lipoic 100 mg significant anti aging
ALC 125 mg + melatonin 40 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20783

ALC 125 mg + lipoic 100 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20729

taurine 1 gm
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20684

The negative result with coconut juice was a big surprise, as this
type of fruit juice has repeatedly exhibited a survival promoting effect
in the past. However the coconut juice used this time is from a different
source, and possibly heat treatment may have deactivated the juice

ALC 125 mg + lipoic 25 mg significant anti aging
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20572

The 1/4 tsp dose of bergamot leaf again increased survival. A
published experiment found that black tea also increased survival due to
the presence of a volatile iron chelator, which prevented iron
accumulation in fly tissues. I suspect that this volatile iron chelator,
which is not present in green tea, may have been derived from the bergamot
leaf, which is added to some black teas. Since I used bergamot leaf
powder, rather than brewing a tea, the leaf powder may have released this
voltaile iron chelator gradually. At any rate, this is one theory
explaining why bergamot appears to work at a low dose. The high dose
might be inducing a marginal iron deficiency.

citrulline 1/2 tsp
bergamot leaf 1/4 tsp significant anti aging
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20421

150 mg of valerian, and 50 mg of melissa(lemon balm).
sumac 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20397

Only sodium chloride offered an advantage, and then only late in life.
Possibly aged flies have impaired immunity, and sodium chloride may have
helped to suppress the growth of any microorganisms that could survive the
mold inhibitor & citric acid combination already incorporated into the fly
food.
sodium chloride 1/32 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20376

higher doses of fresh cinnamon offered a
survival advantage late in life. The higher dosages of paprika also
offered an advantage.
cinnamon, new 1/8 tsp this can be obtained by crushing fresh sticks of cinnamon
paprika, new 1/8 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20338

CDP-choline 250 mg +methylcobalamin 5 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20136

coconut juice FC
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20056

boysenberry FC
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20010

paprika 1/4 tsp +cinnamon 1/4 tsp +soy isoflavones 200 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19962

honey 2 tsp - extreme longevity
everlasting flower 1 tsp
rice, black 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19816


it does appear that tansy, tarragon, and paprika offer a further benefit.
honey 2 tsp + paprika 1/4 tsp
honey and + tarragon 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19756


elderberry FC- extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19489

bergamot leaf 1/4 tsp - extreme longevity
periwinkle 1/4 tsp
celandine herb root only 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19206

Screening for Antimicrobial Activity of Some Medicinal Plants Species of Traditional Chinese Medicine
this shows that celandine is a powerful killer of microrgamisms
The results showed that the extracts from Sanguisorba officinalis, Tussilago farfara (aerial part; rhizome), Chelidonium majus (root), Tribulus terrestris (aerial part) and Schisandra chinensis (leaves) possessed antimicrobial activity.From this study we can conclude that the plants C. majus, S. officinalis, and T. farfara (also called Coltsfoot is used for asthma, bronchial congestion and is one of the most potent cold and flu remedies known)possessed the highest antimicrobial activity. All these species are perennial herbs widely used as medicines and described in the Chinese Materia Medica (B���������� & G���������� 1993).


Sanguisorba officinalis L. (Rosaceae) 0125
Tribulus terrestris L. (Zygophyllaceae) 0156
Tussilago farfara L. (Compositae) 0117


buckthorn bark 1/16 tsp
bergamot leaf 1/4 tsp
buckthorn bark 1/16 tsp
celandine herb 1/4 tsp
celandine symergists with mullein
periwinkle 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19206

onilne herbal manual
http://www.herbaled.org/THM/thm_index.html

walnut/potato 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19134

rice protein 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18927

soy isoflavones 200 mg - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=19017

tansy 1/4 tsp
tarragon 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18247

tansy synergies with garlic and tumeric
The Tansy Rhodiola Eye Cream has worked phenomenally at reducing any puffiness around my eyes

Soy and rice may
exert a small retarding effect on motor neuron degeneration.
soy protein 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18221

pepper, cayenne 1/8 tsp
stevia 1 packette- extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18127
Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana.
It might be adequate to conclude that stevia leaves could protect rats against streptozotocin induced diabetes, reduce the risk of oxidative stress and ameliorate liver and kidney damage
http://www.ncbi.nlm.nih.gov/pubmed/23140911

corn bran 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17845

agar-agar 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17825

elderberry FC - extreme longevity
grape
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17520

Best result was with
balsalmic vinegar, which offered superior survival for the first month.
This vinegar had the darkest colour, and so I presume the highest
phytochemical content. I note that this vinegar is manufactured from grape
juice, and that grape juice has offered survival advantages on several
occasions in the past when I have tested it.
Since in humans potassium depletion is a side effect of excessive
vinegar use, I decided to test the effect of a small nutritional
supplement of potassium. This did appear to restore maximum survival of
white vinegar treated flies to close to control levels. It is interesting
that in a published study, which did not use an added acid, potassium also
improved survival in flies.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17466

vinegar, white 1 tsp + potassium 1/64 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17466

Each grape that will ultimately transform into balsamic vinegar is a tiny gem packed with powerful restorative qualities

endive 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22691

oxymol syrup , old 2 tsp - significant life extendtion
pomegranate, new 1 tsp - significant life extendtion
loosestrife 1 tsp - significant life extendtion
riboflavin 100 mg - significant life extendtion - significant life extendtion
annatto 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22643

loosestrife has a large taproot like the weishtie flower
other plants with large taproots include:


Burdock
� Dandelion
� Kudzu
� Parsnip
� Patterson's Curse
� Poppy mallow
� Radish
� Turnip
� Carrot
� Green alkanet
� Sturt's Desert Pea
� Welwitschia

�http://wiki.answers.com/Q/List_the_tap_root_plants

Food Club dyes yellow dye 1 drop
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22255

oxymol is possibly called Hyssop Oxymel -
how to make Oxymel
http://www.learningherbs.com/news_issue_99.html

spinach 50% in september - significant life extendtion
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22545

broccoli, stem 13% - significant life extendtion
kale, leaves 13%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22524

suey choy 13% - significant life extendtion
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22491

oxymol syrup2 tsp
sour cherry syrup 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22472

coffee/milk kept in the dark - significant life extendtion
wax gourd drink
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22414

Dark-cycle, night administration of the pineal hormone melatonin in drinking water to aging mice (15 months of age) prolongs survival of BALB/c females from 23.8 to 28.1 months and preserves aspects of their youthful state. Similar results were seen in New Zealand Black females beginning at 5 months and C57BL/6 males beginning at 19 months.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC43034/

sour cherry syrup 1 tsp - significant life extendtion
oxymol syrup 1 tsp - significant life extendtion
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22056

In the carob 1/2 tsp bottle some flies retained the ability to fly
after all the flies in the other bottles were dead. This raises the
interesting possibility that although carob might not inhibit viral
mediated mortality, it might inhibit motor neuron degeneration.

carob 1/2 tsp - significant life extendtion
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21992

pomegranate paste 1 tsp - signifcant (month june)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21850


Previous results in run #56 suggest rice protein is active in prolonging
fly lifespan mostly in the absence of citric acid, which had since become
a standard addition, since this acid keeps the fly food "fresh" and
thereby increases fly lifespan. Although at acid pH,
absorption of protein is known to be reduced, I suspect the real reason
citric acid counters rice protein, is that the acid pH destroys the active
ingrediant(s) in rice protein.
rice protein, old 1 tsp - very signifcant
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21771

A maximum longevity of 124 days, with 1/2 tsp pomegranate in the
current run, happens to be an all-time record for my series of
experiments. The previous record of 118 days was set by freeze
concentrated Knudsen elderberry nectar back in run #36.
Follow-up experiments will be reported on, in due course. For now,
I'll just mention the 1/2 tsp pomegranate dose was added to 20 mg of fly
food, which is about 80 calories. Scaling this up to 2000 calories/day,
yields a comparable human dosage of about 4 tablespoons per day.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21734

annatto 1/4 tsp
niacin 100 mg
nicotinamide 94 mg + NAD 2.5 mg - very signifcant efects
The addition of NAD to nicotinamide
did increase longevity,
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21633

Hawthorn Juice
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21507

white gourd Juice 100% - extends lifespan
starfruit Juice - extends lifespan
coconut Juice (Sun &Dragon)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21406


honey suckle 1/2 tsp - extendds lifespan
loosestrife 1/2 tsp extendds lifespan
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21106


honey, buckwheat 2 tsp
malt beverage (Clausthaler) - signifant
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21034

grass jelly drink
white gourd drink
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20938

ALC 125 mg + nicotinamide 94 mg
lipoic 200 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20830

ALC 125 mg + lipoic 100 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=20783

Although late in some of my
experiments I do see some flies losing the ability to fly, due to
age-associated motor neuron degeneration, I believe the bulk of my flies
die from viral infections, rather than from cardio-respiratory arrest due
to extremely advanced age.
In celebration of my 100'th fly experiment, I've decided to retest all
the supplements in duplicate, that produced at least one fly that reached
over 105 days of age in the first 99 experiments. These are always from
experiments conducted during the winter, when indoor temperatures are
lower. The supplements are as follows:
Kambiz pomegranate paste (124 days - run# 87),
Knudsen elderberry nectar [freeze concentrated] (118 days - run# 36)
Sun-Rype apple juice + vit C (107 days -run#36)
Huaqi hawthorn juice (106 days - run# 84)
Taisun Enterprises starfruit juice (106 days - run# 83)
Nu'O'C Bi Dao white gourd juice (106 days - run# 83)
Storage in the dark increased maximum survival in the control, apple,
elderberry, hawthorn, and pomegranate bottles, but reduced it in the
Starfruit, and white gourd ones. Is all this due to chance? (I further
test dark storage in run #101.)

possible apple,elderberry, hawthorn, and pomegranate bottles, are winter seasonal foods

All supplements increased maximum survival. White gourd juice garnered
the maximum survival sweepstake, with a winning score of 88 days. However
hawthorn juice provided more consistent results, and gained the runner-up
position at 82 days. These are just 24%, and 15% above the control maximum
longevity of 71 days.


control 86 86 86 67 62 52 24 5 0 - - - -
+(dark after day 11) [SB] 65 70 50 25 25 10 10 5 5 0 - - -
apple juice 70 70 61 39 39 30 13 9 0 - - - -
+(dark) 79 79 74 63 53 37 32 11 5 5 0 - -
elderberry nectar FC 81 81 81 63 63 63 38 13 6 6 0 - -
+(dark) 94 94 83 72 61 61 50 39 28 11 0 - -
hawthorn juice 88 88 79 75 71 67 42 25 13 13 0 - -
+(dark) 100 100 83 78 78 57 48 48 26 22 4 0 -
pomegranate paste 1/2 tsp 85 80 80 70 60 50 35 15 0 - - - -
+(dark) [old paste] 85 85 85 75 55 55 25 15 10 5 0 - -
starfruit juice,raw 95 79 79 68 58 58 37 21 11 5 0 - -
+(dark) 83 83 67 50 33 25 8 0 - - - - -
white gourd juice 81 81 76 67 48 48 38 24 19 10 10 5 0
+(dark) 88 88 81 31 25 13 0 - - - - - -
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22367

fresh unopneed mixtures get the best results, therefore rosemary adn other preservatives work best.


White gourd juice is gentle on the stomach and is easily digested. A single serving of white gourd juice contains about 70 calories, and 0 grams of fat. This drink is rich in fiber and contains lots of water. It helps if you drink White Gourd Drink in the afternoon, specifically between 2 PM and 3 PM.
http://www.ehow.com/info_8687564_nutrition-white-gourd-drinks.html

annatto 1/4 tsp - signifncat life extention
oxymol 2 tsp - signifcant life extention
pomegranate paste 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22298

In all cases the day 36, and day 41 survivals were
increased with dark storage, though maximum survival was not always
increased. Possibly the elimination of light induced immune suppression,
may have reduced virally mediated mortality. In any case I have decided to
use the dark storage condition as part of my standard protocol for
subsequent runs.

In all these cases there appears to be a
narrow therapeutic range, with effective doses becoming rapidly toxic at
higher doses.



fucoidan 52 mg
menaquinone 1920 mg
taraxatone 1 cap
ribose 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=30264

phylloquinone 1250 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29686

+thiamine 50 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29811

pantethine 300 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29593

palm seed 6.6%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29593

phoshatidylcholine (PC) 105 mg
hawthorn phytosome 50 mg
chrysin 125 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29505

Roasted Barley Extract 1 tsp
goji Berry 125 mg
quercetin 125 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29338

astaxanthin 1mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29128

cyclamate 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28757

deer antler velvet 8 mg
eyebright 118 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28609

Jiao Dasheen starch 2 tsp
silver water 1 drop
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28505

aspartame 8 tabs
saccharin 16 tabs
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28336

dilantin 13 mg - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28270

Imedeen 1 tab
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=28206

terminalia arjuna 500 mg - powerful life extender
wuzi yanzong 100 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27864

pregnenolone 400mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27855
Jiao Dasheen 2 tsp - powerful life extender
rice flour 4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27835

2 tsp of any sugar was more beneficial than the 2 tsp dosage.
Honey 8tsp
sucrose 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27792

phaseolamin 31 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27766

fumaric acid 125 mg - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27682

cyclamate 1tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27655

dilantin 13 mg - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27638

aged garlic 150 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27582

ginkgo biloba 10 mg - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27544

cantalope 5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27523

honey 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27483

lavender 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27483

fructus lycii 2% - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27459

Jiao dasheen starch 1/2 tsp - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27457

honeysuckle 1 tsp - powerful life extender
methionine 125 mg(low doses work best)
bearlic 75 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27306

In run #8 flies fed Kyolic Formula 100 aged garlic extract at a 75 mg dosage outlived the controls by a few days.
forskolin 2.5 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27137

foo-ti-teng 130 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27119

diindolymethane 25 mg - powerful life extender
terminalia arjuna 125 mg - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27107

trisnake 1 pill - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=27002

TriSnake Pills formula

Fan Pi
Cang Zhu
Bai Hua She
Wu Shao She
Mu Dan Pi
She Chuang Zi
Dang Gui
Ren Shen
http://www.cryonet.org/cgi-bin/search.cgi?sort=new&sort=new&sstr=fly+longevity+experiments&start=61

phylloquinone 2560 mcg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26961

urea 1/16 tsp
vinpocetine 30 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26919

gymnema sylvestre50 mg
docosahexaenoic 17 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26899

pomegranate juice 40% interpret this as implying that pomegranate probably
exerts no anti-aging effect as such, but instead can help reduce pathogen
associated mortality, and so increase the likelyhood that some (fragile)
flies can live to an extreme old age.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26881

bacillus natto 1/4 tsp - powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26837

citric (1/4) 0 tsp 83 54 46 25 17 4 4 4
citric acid should not be used long term
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26801

blue-greenalgae 1/4 tsp- powerful life extender
chlorella 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26767

cerium oxide nanoparticles 1/16 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26367

vinpocetine 60 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26344

bacopa monniera 75 mg powerful life extender synergists to be located
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26320

triphala 250 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26294

bacillus natto 1/2 tsp powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26427

salix alba 25 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26253

choline alfoscerate 63 mg
pantothenic acid 63 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26114

hyaluronic acid 45 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26082

20% cranberry
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26058

broccoli sprouts 2.5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25424

lactobacillus: 16 mil powerful life extender
95% L rhamnosus (killed) 3 mil
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24991

black sesame between 25 and 50%
Low dose PEG may have offered some small net benefit to old fliespowerful life extender
PEG 10,000 1/8 tsp

red cabbage (extract) 50% powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24749

sunburst squash 5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24730

Here I repeat run #75, which examined the effect of fly number per
bottle on longevity. That experiment found that longevity was best with
fly numbers below 10,
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21798

elderberry 200%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16020

citric 1/4 1/8
As can be seen, joint addition of
citric acid to both the breed and run bottles increased maximum
longevity in the present experiment by up to 52%.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25663

salix alba 50 mg powerful life extender synergists to be located
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25602

50 mgsalix albapowerful life extender synergists to be located
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=26253

marmite yeast ext 1/2 tsp extremly powerfu life extending properties
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24018

For some time I have been adding 1/8 tsp citric acid to all bottles,
despite previous tests indicating that higher doses were more beneficial.
This has resulted in some false positive results, when an added supplement
provided addition acid. I had been concerned that many phytochemicals
would be less stable at acid pH, hence the conservative use of citric
acid. However I have since learned that some plant phytochemicals are
more, not less stable at acid pH.
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24018

pomegranate paste 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21850

citric 1/4 1/8
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25663
In order to boost maximum longevity citric acid has to
be present in both the breed, as well as the run bottle

tyrosine 15 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25714

Foo-Ti-Teng 130 mg- powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25897

honeybush 4 and / or 8 bag/L- powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25820

arugula 5%- powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=25751

huperzine 2 mg
inositol 2 tsp powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24626

"Longevity-enhancing Effects of Bacterial Exposure in
Fruit Flies "
(
http://www.bioscienceworld.ca/view.html?id=361)
"Bacterial exposure can increase longevity, but only
when it occurs early in life

tyrosine and cinnamon 1/4 tsp powerful life extender
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24598

hyacinth bean 5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24544

In Run #18 I found that survival was better with 1/4 tsp citric acid
added to 20 gm 4-24 fly food, than 1/8 tsp.
elderberry 200% - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=16020

For now,
I'll just mention the 1/2 tsp pomegranate dose was added to 20 mg of fly
food, which is about 80 calories. Scaling this up to 2000 calories/day,
yields a comparable human dosage of about 4 tablespoons per day.
1/2 tsp pomegrante paste
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21734

rice protein 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18334

white gourd Juice 100%
starfruit Juice
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=21406

Longest Living Animal? Clam -- 400 Years Old -- Found In Icelandic Waters
ScienceDaily
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=29985

lettuce, head 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22746

corn bran 2 tsp
arabica gum1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17845

I believe virus induced
mortality to be a major factor in fly longevity in the summer, unless
freeze concentrated elderberry nectar is used in place of water. This would mean taking more elderberry in summer is key.
agar-agar 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17825

pollen allergy (1 cap)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24280

soy protein 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18221

sterols 432 mg - extreme longevity
tea leaf,OrangePekoe 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23628

stevia 1 packette - extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18127

freeze concentrated elderberry juice extreme longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=17520

tansy 1/4 tsp
tarragon 1/4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=18247

Marmite and Rosemary may work well
lactobacillus 32 bil cells - signifncant anti aging
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24490

sucrose 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24425

citric 1/2 tsp
sucrose 2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24368

However
inexpensive common table sugar (sucrose) was effective as well
lysine 1 gm extrem longevity
tyrosine 4 gm- extrem longevity
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24307

oyster sauce 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24280

glucose 2 tsp - better longer lives than sucrose
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24260

purple sweet potato 20% (also called purple yam)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24200

urad bean 5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24110

great northern bean 5%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24071

marmite yeast ext 1/2 tsp - strong anti aging effects
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=24018

nori seaweed 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23924

coffee decaf 4 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23838

Orange-Pekoe leaf 1/2 tsp
oolong leaf 1/2 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23768

artichoke powder 1620 mg - benefifcal anti aging effets
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23704

lentils, French 20%
sterols 432 mg
Even at 99 days of age, some sterol fed
flies could still take flight, which is an anomaly I have never before
observed. (Sterols used were New Roots brand)
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23628

Sterols and carnosine to be looked into

flageolet beans 5%
red ginseng drink 100%
raw pomegrante innefective in spring
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23572

lentils, French 4%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23538

plantain banana 20%
kidney beans, white 12%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23489


In all of my previous
experiments only one fly fed some pomegranate paste in run #87 lived
longer - 124 days.
rooibos chips 1/2 tsp
grape,black,pulp 8%
carob 1 tsp
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23418

carob and yogurt to be looked at

he resveratrol in dried peanuts is known to remain stable for
years.
peanut skin 2 tsp - extreme longevity observed
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23366

kidney, white 8%
theanine 100 mg
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23285

Yellow banana pulp once again offered substantial protection during
the first two weeks of the experiment
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23229

banana, red,ripe 8% - extreme longevity
dragonfruit, pulp 20%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23226

nectarine 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23184

yam 8%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23151

onion, red 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23120

leek leaves 8% (toxic?)
mushroom, Portabello 8%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=23058

tomato, red 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22988

carob 1 tsp
cantalope 33%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22890

skim milk 10%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22811

squash, acorn 33%
lettuce, head 8%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22746

apricot 33% - extreme longevity
endive 33%
mint 8%
http://www.cryonet.org/cgi-bin/dsp.cgi?msg=22691

In-vitro tests show that Acetyl Tetra peptide-2 increased cellular growth (as measured in keratinocyte density) by 51% in just 5 days! In addition, it enhanced the metabolic activity of keratinocytes, increasing the production of keratin by 75% and of keratohyalin by 28%.

http://www.lef.org/magazine/mag2013/jun2013_Unique-PePtide-Repairs-Aging-Skin_01.htm

Wheat, Eggs and Soybeans are high in Peptides

 

 

Please note this is a scientific work in progress so the font, text and outlaying are not going to be perfect. At this stage information mining and verification is key. Perfection comes later�

Books:
Rasayana:
Ayurvedic Herbs for Longevity and Rejuvenation

Internet Guide to Anti-Aging and Longevity





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